XB-CLONE-3569306
Clone Name: IMAGE:7706866
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Gene:
taf1d
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC156032 [+]
CGCAGCAATGTTTGAGGAAGGAAGTGAACCAGATGCACACAGCTATGATATTCTTCAAAGCCAAGAACTTTTTAGTGATTCGGATTGTAATTTATTAACG
AAGGATGCCAGTAAGCCAAACAAAAGTCCAACTAACAGTCATCATGGCCGTCTGCTGGACAGCTGTTCTGAATCCAGTAGTAGCCTATTCCAGACTGAAA
TTACCTGCACACCTTCAAGAACCAGGAGACGACAAACTGCAAATAGACCCAAACCCTTGTCACCACTGTCATCTTTATCTGACTCCTCAGACTTTGAACC
ATTACCAAAGAATGTCCCTTTGGAAAAGATCTTTGAGCGGTTCAGGAAAATGAAAAAAAAACATAAGAGAAAAAGACAGAAAAAGAAGTACAGAAAGCAT
TTAATTACAGAAAAGAAACCCAAGAAACCTCCAACAAAAAAGCTCACTATTCCTTTGCAAGAAAAGTATCGCCGACTTAAAGAAAGAACAGTTGAGTTCC
CTTTTACTGGCCAAAAATATTTGCATTTTAAACGGTATCTTCCTTATGAGCAATCTGTTTTTGGTGGATTTCTGAGCCATATTGAAAAGCTTAAGTGTGA
TCGACATCTGCAAAATTCATTACAGGGCATGGATTTAGGGGAAGATTTAGAGAATGAAAGTTTAGAAATGCATAAATATGGGTACCTGGATGATGCTGGA
CCGATTTCACCTATCGCTGAGCACGATGCGAATACTACCAGTAATGAAGGTCTGTGTAATTATGATGCAAAAATTGTGGAAAATGACTCATTTATATTAA
AATACAGTGTGCCAAGCAAGGGAAAATGGTGTGTTAAAAGGAAGAAGAAAATTAATACATTGAATTCTGAACCAAACAACCTTAATTTCCAGGAAGATGG
ACAAGTCTGAGTCAGACAAATGAAAACCATGTTTTCCAGGGTTTTGAGAGAGCACATTCAAGGCCCATGGCCTCTTCATAAAAAATTGTTTTGAAGCAAA
ACAAATTGTACAGATTGCAAAGTAATATATTTTTATACTACATTAATCATTCAAATAAAGTTTAGGAATATTTAAAAAAAAAAAAAAAAAAAAA
5' EST: CX959225 [+]cgcagcaatgtttgaggaaggaagtgaaccagatgcacacagctatgatattcttcaaagccaagaactttttagtgattcggattgtaatttattaacg
aaggatgccagtaagccaaacaaaagtccaactaacagtcatcatggccgtctgctggacagctgttctgaatccagtagtagcctattccagactgaaa
ttacctgcacaccttcaagaaccaggagacgacaaactgcaaatagacccaaacccttgtcaccactgtcatctttatctgactcctcagactttgaacc
attaccaaagaatgtccctttggaaaagatctttgagcggttcaggaaaatgaaaaaaaaacataagagaaaaagacagaaaaagaagtacagaaagcat
ttaattacagaaaagaaacccaagaaacctccaacaaaaaagctcactattcctttgcaagaaaagtatcgccgacttaaagaaagaacagttgagttcc
cttttactggccaaaaatatttgcattttaaacggtatcttccttatgagcaatctgtttttggtggatttctgagccatattgaaaagcttaagtgtga
tcgacatctgcaaaattcattacagggcatggatttaggggaagatttagagaatgaaagtttagaaatgcataaatatgggtacctggatgatgctgga
ccgatttcacctatcgctgagcacgatgcgaatactaccagtaatgaaggtctgtgtaattatgatgcaaaaattgtggaaaatgactcatttatattaa
aatacagtgtgccaagcaagggaaaaatgtgtgttaaagga
3' EST: CX959224 [+]aaatattcctaaactttatttgaatgattaatgtagtataaaaatatattactttgcaatctgtacaatttgttttgcttcaaaacaattttttatgaag
aggccatgggccttgaatgtgctctctcaaaaccctggaaaacatggttttcatttgtctgactcagacttgtccatcttcctggaaattaaggttgttt
ggttcagaattcaatgtattaattttcttcttccttttaacacaccattttcccttgcttggcacactgtattttaatataaatgagtcattttccacaa
tttttgcatcataattacacagaccttcattactggtagtattcgcatcgtgctcagcgataggtgaaatcggtccagcatcatccaggtacccatattt
atgcatttctaaactttcattctctaaatcttcccctaaatccatgccctgtaatgaattttgcagatgtcgatcacacttaagcttttcaatatggctc
agaaatccaccaaaaacagattgctcataaggaagataccgtttaaaatgcaaatatttttggccagtaaaagggaactcaactgttctttctttaagtc
ggcgatacttttcttgcaaaggaatagtgagcttttttgttggaggtttc
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
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