XB-CLONE-3567689
Clone Name: IMAGE:7707954
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Gene:
bud13
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC161419 [+]
gtagtaatggcagcgcccggtgctaaggtgcagtctctgtctaaagctgaatacttgaagcggtatctagattctggaggcgacaatggggaggcaaaga
aaaagaagaaaaagagtaagaggacagaaggaaaggggatgcggatagttgacgatgatgctgaagagtggaagaagatgcctagcaaacaagagcaagc
ggtagaggtagaggaagaggaggatcagcctgttgtggcagaattcgtagatgaacgacctgatgatgtaaaacaaatggagatattcaggaccagcaac
aaatggaaactaattcaagatactggaaatgaatcatccaggatggagagttcttcagctcttgtatccatagacggtagtactgacaggccaactagtt
caccgaaaagaaatattcagaattatagagagaaagatgattcttctgatgcctccccaccttgcagaaagaggcatgattcttcagatccatcacctcc
tccaagaaaaagacaagattcttctgataaatcactacctaggagagggaggcacaactctaccgatccgtctccaccccggagagggagacacgcctct
cccaacccttctccaccccggagagggaggcacgcctctcccggcccttccccaccccggagagggaggcacgcctctcccggcccttctccaccccaga
gagggaggcacgactctcccgacccttctccaccccggagagggaggcacgactctcccgacccttcgccaccccggaaagggaggcacgactctcccga
cccttcgccaccccggagagggaggcacgactctcccgacccttcgccaccccggagagggaggcacgactctcccgacccttcgccaccccggagaggg
aggcacgactctcccgacccttcgccaccccggagagggaggcacgactctcccgacccttcgccaccccggagagggaggcacgactctcccgaccctt
cgccaccccggagagggaggcacgactctcccgacccttcgccaccccggagagggaggcacgactctcccgacccatctccaccccggagagggaggca
cgactctcttgacccttctgtaaatcgaaaaagaaaacaaggttctactgaaccttcattttctagaaataaagaatgtaaatatccttctgatcccttt
gcaactagtaaaagtaggcagaactcaagtaaatcttctccctctcaaaaagattatcacagctcatcaaagatcatgcctcaccacaaggatcagtata
aatcatctgcacactcatcccgcactctgaagggcggagaaaaaactaagaatttctcatctcatgacaagggaaaaattgactcccaggagctttccag
caacttgtctgagaaggaccgtaaacgtagacatgatatttacaaacctacaaattcaaacagaagtgcacagtgccaaaaccttaatagtttcaatcac
cattcctctgctagacacaattctgactcagaatcccctcattcaaagaaaagagacaagacacgaaaaagaaggaaagaagcaacacagcagcagaact
ctgattcagacctttctccaccacgtcacacaaagaaaagaggctcagactcagacctgtcaccaccacggcatacacgaaaggactcagattctgacct
ctccccaccacgtcactccaagaaaaggggttcagactcagacctttccccaccaaggtgtagaactgatgaccacagaggctctgcagttggtcgcata
caaaaaggagaaagttctaggatgctgtctggaggcagagtcggccttgtgtctgctgagatacttagggaggaaaaggtggagctgagaaagggactaa
aagataacaaacatttggaagatgaatccaggaatgcagaaactgttttcagagataaaagtggcaagcgcagggacctgaatatagaacgggaacaaca
gcaaaaaaaattggaggatgctatcaaagaaatggaaaagccacttgccagatacattgatgatactgatctggacaaaatgttgcgagaacaggagaga
gagggtgacccaatggccaaatttctgcaaaaaaaaaaaaaaaaaa
5' EST: CX961575 [+]tagtaatggcagcgcccggtgctaaggtgcagtctctgtctaaagctgaatacttgaagcggtatctagattctggaggcgacaatggggaggcaaagaa
aaagaagaaaaagagtaagaggacagaaggaaaggggatgcggatagttgacgatgatgctgaagagtggaagaagatgcctagcaaacaagagcaagcg
gtagaggtagaggaagaggaggatcagcctgttgtggcagaattcgtagatgaacgacctgatgatgtaaaacaaatggagatattcaggaccagcaaca
aatggaaactaattcaagatactggaaatgaatcatccaggatggagagttcttcagctcttgtatccatagacggtagtactgacaggccaactagttc
accgaaaagaaatattcagaattatagagagaaagatgattcttctgatgcctccccaccttgcagaaagaggcatgattcttcagatccatcacctcct
ccaagaaaaagacaagattcttctgataaatcactacctaggagagggaggcacaactctaccgatccgtctccaccccggagagggagacacgcctctc
ccaacccttctccaccccggagagggaggcacgcctctcccggcccttccccaccccggagagggaggcacgcctctcccggcccttctccaccccagag
agggaggcacgactctcccgacccttctccaccccggagagggaggcacgactctcccgacccttcggcaccccggaaagggaggcacgactctcc
3' EST: CX961574 [+]gcagaaatttggccattgggtcaccctctctctcctgttctcgcaacattttgtccagatcagtatcatcaatgtatctggcaagtggcttttccatttc
tttgatagcatcctccaattttttttgctgttgttcccgttctatattcaggtccctgcgcttgccacttttatctctgaaaacagtttctgcattcctg
gattcatcttccaaatgtttgttatcttttagtccctttctcagctccaccttttcctccctaagtatctcagcagacacaaggccgactctgcctccag
acagcatcctagaactttctcctttttgtatgcgaccaactgcagagcctctgtggtcatcagttctacaccttggtggggaaaggtctgagtctgaacc
ccttttcttggagtgacgtggtggggagaggtcagaatctgagtcctttcgtgtatgccgtggtggtgacaggtctgagtctgagcctcttttctttgtg
tgacgtggtggagaaaggtctgaatcagagttctgctgctgtgttgcttctttccttctttttcgtgtcttgtctcttttctttgaatgaggggattctg
agtcagaattgtgtctagcagaggaatggtgattgaaactattaaggttttggcactgtgcacttctgtttgaatttgtaggtttgtaaatatcatgtct
acgttta
Library:
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropTe3 and Te4 libraries). Library constructed at the DOE Joint Genome Institute.Anatomy: testis | Stage: frog |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Suppliers: