XB-CLONE-3565939
Clone Name: IMAGE:7709795
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Gene:
cnbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964561 [+]
ttttccccttcctcctccagctttattgatagtttaaaattacatttctatgttctaggacttaagtttctttttacctcctgactttaaaaactgattc
caagcaaagtaaactcaaacagttgcctaaagtgatatcgtatacaaaaataactcttgattctgtgaaaacatcgtttctctgcagctcccgaacagct
tctacgtttggcttctctccgccaggcgcctcctcgccgttagcgctccccgtaaagaaacaagaaaacgaaataaaaaataaacgtattcgcatcctgg
cttttgcctcatctccaatagcaacgcggtggccaagcgtcggcttcgggcgggttcagatgcgacagggccactggctgacgcgtgactctcgctgtag
acaggcccgagacaggtgtggatgaacagccaatgagactgtcacaaacgcaaagttgcgcagaaactcccaatagcttttccccgcccccgaggagcag
gagggctgccgaatacctgcccgggggccgactcttcccattgaattccctattcaccagtggcacggaaagggggttctttaacaaagcattatacata
acacctctaccaaactaaacataaacatttttttttttgtagtttaaatgcagaaagtcattttgtgtttccacccctttcctccttttacacgtcaagt
aaagctcacggccggngaatagctctatctgccaacctttggccagtgaagaggactcagagaaaagaatacaaccatncatc
3' EST: CX964560 [+]catttgactgtagctcactagtaagaaaggttggggacccctgtggtaaagcgttagtgagcccgcccccttagtgtaagccccgcctcattatccttat
cgttcccaggtgtggggagaccggccacgtggccatcaactgcagcaaaacgagtgaagtcaactgttaccgctgcggggagtcggggcaccttgcgcgg
gaatgcacgatcgaagccacggcctaaatatccttcgtcgcccctcctttttctgattgatggttgtattcttttctctgagtcctcttcactggccaaa
ggttggcagatagagctattccccggccgtgagctttacttgacgtgtaaaaggaggaaaggggtggaaacacaaaatgactttctgcatttaaactaca
aaaaaaaaaatgtttatgtttagtttggtagaggtgttatgtataatgctttgttaaagaaccccctttccgtgccactggtgaatagggaattcaatgg
gaagagtcggcccccgggcaggtattcggcagccctcctgctcctcgggggcggggaaaagctattgggagtttctgcgcaactttgcgtttgtgacagt
ctcattggctgttcatccacacctgtctcgggcctgtctacagcgagagtcacgcgtcagccagtggccctgtcgcatctgaacccgcccgaagccgacg
cttggccaccgcgttgctattggagatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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