XB-CLONE-3565912
Clone Name: IMAGE:7709559
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Gene:
eif4b
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964579 [+]
gaatccatccagaagttctttagggggcttaatataagtgctgttcgtctaccccgcgagccaagcaatccagagaggttaaaaggctttggttatgcag
aatttgatgacttggattctctactacgggctttaagcctcaatgaggaatctcttgggaacaggaggataagagtggacattgctgatcaggcacaaga
taaagaccgggatgagcgctcatttggtagggatcgtgatcgcaacagagagtcggacaagacagattcttctgattggagggcgcggcctagcacagac
agctttgatgattatcctccacgtcgtggagatgacagccagggggacagatatcgcagtgaccgctatgaatcagaccgttaccgtgatggtccaaggc
gcgatggagaccgctatgaaagggacaggggtagagatcggtatgatgatcgtaatcgaagtgattatgatagaggctatgattcccggggcggcaggag
ggcctttgggagtggctatcgtcgagatgatgattacagaggtggtggaggtggtggagatcgttatggagatagatacgaggaaagacgagaggacaga
tcagacagatggaatggatattctcgcaatgaagagaggcgggaagaacgagcgggtcccactcagcgaccaaaactaaacctaaaaccacgtactgctc
caaaagatgaggaaggtgggccatctgccgccaccagtcgtgcagcttcaatttttggtggtgcaaagccagtagatacagctgctcgtgaacgagaagt
ag
3' EST: CX964578 [+]ttagagggctggtaagggggatctttacatgataataaaacatctcccagaaagcagattacaaatcattacaagttttattatatatttcccttgattt
acacttctgcgtgaagagtaaaattgctagggaaggtatcgcaagctggtccatgagagacagacaggaacacagctcctggatgcacatcaattataaa
ttagggtaggccgagtgaaggggattggagaggtgcaatttgagctcaggaaattgtcaaatttggtatgaaaggcagtgtcccatagctgcgtgccatg
caaacgctgcctaacccggattccttatatattctaataaactctgcataatatagccttccaactgatgaaaatatcaccgctacagcagtctctttca
cacccacagcatcacacaaagccggaggggggaatttcctaaaaaagcaattaatatttgaatccctgacaagggatgcggcttctttaattgatcattt
tctctggcacacggaggtgggcttctggcaaatgctctagaagagtgcaaaccatgcatctgatgcccttgtgaagctgctaaataagtcttcctccggt
catatgatttgcctagccatactgtactaaagggcatttctttcattggagaataatcctaaacagagagctctagcagccaccttgtccagtttccagg
ccactttgctccatatcaagcaaaaatgactgttctggcataaaatggaaactttatacagcanggcagataggatgtgagaatcctttactcatcttac
acaactactagtttcctta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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