XB-CLONE-3565897
Clone Name: IMAGE:7709799
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Gene:
slc13a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964589 [+]
ccgaggttttttgttacaatacttaatgatgtatttgcattagtaacatgtagctcattgagctctgaaacctagggcacctagatatttcacccaatac
tgatagagccattaaaatgtttacattatttttcttaaataaatacaagtgagatacttgccctgatacaaaaaattaagttcttagagagaacttttta
cactcttgcctgctgggtattgcccttattaacagtactattaactgataaggtccccaattaaaagctttgcagagcagagtgagatgctaccttgact
attctgtaggccggtagggtactcggcccatctgcttatagtcacagggtgggcacggcttgtgcgtattcacggacactagagagtctgaactgtcaat
ctgtggattctggcagaagtcagtggggctgctgtaagatgccataaagaatcactgtttattgggctgatgggggactgtttgagtctcttggtagttg
aaatgccagggtctattttgaattccagtctgaacctgtgccacatccaccactttatcatacttctgaggctccttggcatccagttaaacacaactgg
ccattaacagtccattacaaatgctaaggctgccctgtggagtttgcccaggatggaaatgtgcccaagtcgaacatgtaaaatccccagctgttaatgg
ccagtgttatagtcagcacacccagaatgttgagcagtagtcctgcctttgccatgtcgataaccttgagctgcccataagagaaggcaatggcattggg
tggcgttgcaactggcagcataaaagccaagg
3' EST: CX964588 [+]tcctctctttcttctgcccctgcccctggtagtgcccaccaaggaggcaagctgcggctttgtgatcattgtgatggcgttattctggtgcacagaagct
cttcctctggccgtcactgcgctttttccagtcttgctttttccaatgatgggcatcatggattccacagcggtgtgcagccagtatctgaaagacacga
acatgctgttcattggcgggctgctggttgcgatatcggtggagaaatggaatttgcataaacgcattgccctaagagtgctgctgattgttggagttaa
acctgccctcctgcttcttggattcatggtggtcacagccttcctttccatgtggatcagtaacacagccacaacggccatgatgatacctattgcacag
gctgtgctgcagcagcttcacacctcggaagagagagtggacaagccgtcagaagaaacaaaaaagaatgtgaatggaatggataatagcatgtatgaat
cagtattgccaaatgggaaaatgatcctggcagtagaaaacacttatgctactgtaaacgagggatttgagatgcaagatagtactaaagatcctgagcc
ttccaagctagaaaaagaaagtattggcccaatagtgatagaacttgaggatgaaaaacaaaaggaagagaagcagaaacttagattggagaagcacctg
aagatctgcaaaggaatgacccttagtgtctgttattctgccagcattggtggaattgcaacattaactggcacta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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