XB-CLONE-3565894
Clone Name: IMAGE:7709465
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Gene:
csnk1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964591 [+]
gaacgcggatattcttgtccctaagccgaaggatctgctgttctcactactagtcgcatatctctgtttgactccattgtaattgttctgctttatcgct
ggggatccctcccccttccaccccggatcatggcgagtagcagcggctccaaagctgagtttattgtcggtgggaaatacaagcttgtgcggaagatcgg
atctggctcttttggagacatttacctggcgatcaatatcaccaatggcgaggaagtagctgtaaagctggaatcacagaaagctcgccacccccagttg
ttgtatgaaagcaaattgtacaagatacttcaaggaggtgttggaatccctcatatccggtggtatggacaagaaaaggattataatgttctagttatgg
acctgcttggacccagtcttgaggatctcttcaatttttgctcgcgtaggttcacaatgaagacagtacttatgttagcggatcagatgataagtagaat
tgaatatgtacacacaaagaattttatacacagagacattaaaccagacaacttcctaatgggcatcggacgtcactgtaataagttattccttatagac
tttggtttggccaaaaagtacagagacaacaggacgaggcagcacataccttacagagaagacaaaaatcttactggcacggctcgctatgctagtataa
atgctcacctaggaattgagcaaagtcgccgtgatgatatggagtctctagggtacgtgctaatgtatttcaacagaaccagccttccatggcagggata
aaggcagctacaaagagcaaaatatgaaagatagtgaaaagaaatgt
3' EST: CX964590 [+]gtggattaaattttagtttttaatgaacatgtaaacaaacaaaaatgttcaaaataaaaacaaaggtatcccagatcatgtttacagtgattacatttcc
taagccacttaaacaagctttagttgtaagatgttatctagattgtgaacaatatgctgcagtttttccttttacaacatgaacagttaaattccattct
ctaaagaaaagcaagctaagctgtgtcttatgtctgcaccctaacttatcctttaaaaaaagaaaaacaaaaaacacagtcatatggtttgcctaaaaac
tgtaatctttggttaaattaaggagacaagatggtttcagactaccaagatctggcaaagataatttattttctattacactgtttaggagacaagctcc
taaacatgagctgcagtgccacaaggtccaaataatccgaaaccatgcggtcaccataataaccataaggtggcaaaaggaaaaacaaaaaatgatttaa
ctcattcactgaaggtgcacctctaccagactgtagatattttaaacagtgaaacagttaagaatgttttaagtgtacaattaaaaaaaggtgataacga
gtgaagaaaagagaaatgcggaaacaatgagagttttgtttcttttatacaatggcctttaggaaaccagctcatgcctttcttctcaagtagtagaagt
cttaagcagtgtaaggatcaggaggttaaccatagtaaaacgaaaaaaaaaaaccaaaaaaaattggcatatgcacaatttcttccacaagtctgccaac
acttaatatctgaaccacctgtcccacgctttcggggaggggggagggaacgcatagaaaactggacaccatgtttcactaatcttag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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