XB-CLONE-3565885
Clone Name: IMAGE:7709657
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Gene:
ilf3
,
rpl3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964597 [+]
cgttccaagagacatcgtggcaaagtgaagagcttccctaaggatgaccagagcaagcccattcacctgactgccttcctgggctacaaggctggcatga
cccacattgtccgggaggtggacaggcctggttctaaggtgaataagaaagaagttgtggaagcagttactgtggttgaaacacctcccatggtaatagt
tggtattgttggctacgtccagactccccgtggtctgcgcagtttgaagaccatctttgctgagcacatcagtgatgaatgcaagaggcgcttttacaag
aactgctcctctcagctacagtctgatgagctccaggctccgcttgctggctgaaaacctcctttgacgccatgcaatgagccaaaaatctttccttggt
tgggcctcttccgctcatgatcccattatggggcatgatgaagggcctttccaactcttctgatctgccattaaaggtacaaatccaagaagaaggcatt
cacaaagtactgcaagaaatggcaggatgacgagggcaagaagcagctggagaaggactttgccagcatgaaaaagtactgccaagtcatcagggttatt
gcccatacacagatgcgcctgcttcccctgcgccagaagaaatctcacctcatggaaattcaagtgaacggaggtacagtagctgagaaggtcgactggg
cccgtga
3' EST: CX964596 [+]tccagatactgcctgaatgatacgagacatacaaaaaaagaacgcatcccactgtattcttaaaataaattcttcacttataaaacggggaaagaacaaa
aatgagggaaaaaaacaaaaaacatttcaaaacactaacgcgttaaaatacataaagcttgttaagtcacttcacagaattattttaacgatagggaaat
ggaggtgaaaatggatgaatgttctgctcaggattaaaacttcccttacctagagaacaggctaaataccctctctcccagcctctgcgcgcagcagtga
aagtgccaaatgcaagcgcgggacagtgaaaatggcgccacagagccgttaaaccagtcggggggttagtgcaaaaccttatccgcttgtgtttggggtt
aggatttggcttagagtgtacaaatcctaagagcagaaaggttctggcagccatgtttaaaccttccttgacttccacacgtcactatggttccaagttg
aacaagtacaggacaagggcggaaggaaaccaaacttacacactacagacaaggctgcttccgggcgcggtggagagttccgacaatatggcggagggac
agatttgaggagaaagtggaagagaccgtttagaagggagcagagtgaggccgttacataaaagcactgcaacaaggaggagggggaagagcagaacatc
cccttaacgatgtcttanaacgagaagtggtaagtaacaaaagcttggggctcccctcgcagggagactgggggggctagagagtttatctgtactggta
gctcatgttgtggtctgtattcctgccgtaaccacctgctcctccctgaggcgcttggtaattatttggggggcactgtagttctgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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