XB-CLONE-3565684
Clone Name: IMAGE:7709723
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Gene:
eef1a1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964733 [+]
atttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctccttgcctagactctgggagtaaagcaactgatcgttggtgttaacaaaatg
gattcaactgaacccccatacagccagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgatactgttg
cctttgtacctatttctggatggaacggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagagggatc
tggcagcggaactaccctgctggaagctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtctacaaa
attggcggtattggtactgtaccagttggtcgtgtggagactggtgtccttaagccaggcatggtggttacttttgcccctgttaatgtaacaactgaag
ttaaatctgttgaaatgcaccatgaagcccttagcgaggccatgcccggtgacaatgttggctttaacgtgaaaaacgtttctgtgaaggacgtccgtcg
tggcaacgttgctggtgacagcaagaatgacccaccaatggaagctggtacctttacagcacaggttatcatcctgaaccacccaggccagattggtgct
ggatatgcccctgtgttggattgccacacagctcacattgcttgcaagtttgctgagctgaaggaaaagattgatcgccgttctg
3' EST: CX964732 [+]ggtcagttgcttccatttagaaattactggatttaaaacactaataacttaaaaactgccacaaacaaatgtgtccacgagcagttttttccttctgaag
cttttgcgatgcattgtaatcattaaccagtctttgactattaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggtta
tgactgaggtgagagatgctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgc
cttgatgactcctacagcaacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcct
gggatcatgtcaacaatggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcag
caaacttgcaagcaatgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctggcctgggtggttcaggatgataacctgtgctgtaaa
ggtaccagcttccattggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtccttcacagaaacgtttttcacgttaaagccaacattg
tcaccgggcatggcctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgttacattaacaggggcaaaagtaaccaccatgcctggct
taaggacaccagtctccacacgaccaactggtacagtaccaataccgccaattttgtagacatcctgcagaggcaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: