XB-CLONE-3565678
Clone Name: IMAGE:7709819
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Gene:
rassf3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964737 [+]
gaacaccctctgtatctcagactggtagctgggcccagcctcgaagctctcagctttgttttgcgtgagcatgaaaccggagatgttatgtgggaagcct
tcacactacccgagctgcagaacttcttgcgcatattagacaaagaagaagacgagcagttgcagagtttaaaaagacgttacacatcatacagggcaaa
gttagaagaggctttgagtggagtgcacaatcctggctaaaacctaggcaatggagcttctaatgtggtcttctgttacagtattcagcaacagggacaa
aggtgtgcagctctgttcaacagactcttactggggactcgtccctttagagaaggcggatctataaggtgtataccaacactcatcaggacactgcaaa
tgaccactacttaggtggtgcaatagacaatctaaaaaccgaaatcatttttacatgtttttattttttaattttcatgtaaattattacagctttaagt
ctgctaaggtttcttaagactttgttacaaagtcagaaactggtatttatataaattcatgtttctaaagagaatgtttttgtgatgttgcctacgtgaa
tttggtcatgctgtcaggtaagcaaggaacctgaagattcgtaagaattcatgaaggttatgaagcgggaatagttcagtggtatctggtatgttcatat
gctgagatgtgaggcagaaagcatagtaaaatattttggattggtgctgggaagtctcattttaagatggattaagacagagcactgtgcagatatgtat
ctatgcagtgtaggcaaaatgctatgacaggttattttctgccctgttcactgct
3' EST: CX964736 [+]agaaattatattgatcatgaacattacattgtcaaaatatacgaacagtactgtcaactgcaaatgtaataataactgagccgagacttactctgggaaa
tcaattccctataaaaaataaaattttaaacttctttaaaattataaatattataactattaaaactgcgctttaaatgcaccccaaaatcttacttctc
tttcttctcctgtaccatcaaatagtatatattagaaataatattgcatcacaatttttatttcagtatttaagaccttaattgcagctgagaatgctgg
ctaattattcccaatgcccgaatccatttgtgttgccaatattaccagtgtgttcaatcttatcgcttgtgcagagtaagttaatttccagtgcgaattt
ctgctgcatagatcatctcacatagtgaagcacactggcattgcagaggaccatgggtaagacattcagttaatgtggggcactataaaaatggctttgt
ctcattaaaggtaaaaccaggactgtgtacatgaacaggtcaatacagtactttgcccctatccgatgacatgtagtgcatagaatgagctttatgatga
attatccaatatgccatagtcaacttcaagtaaaaaaaataaaataaaatttcacagttgatatttctataaaatataggttgtagcaacagtctgctta
gcctgcttaagaaatgaactcctggattaggcatgcaaataaaccataatatccttccctccctgagccaatttttctttagcaaagcgatattctttca
ctgccacggcagcttcatgtgcagaatagtgagcataaacgataaatggt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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