XB-CLONE-3565669
Clone Name: IMAGE:7709581
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Gene:
acot2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC158300 [+]
cgattcgtttccataagctcctgttatttaaaggaaaatgctggcatctagattgaaagttatgttctgccttctgcgcaggcccctgcctagattcctg
cctgcctgtgccatgtctgtgtctctgcaggtcaccccagggcactgcctgtatgatgagcccctacgggtggctgcttctggcataggacctgggcagg
aagtgaccctccaggcaacagtgactgatgaaggtggggaaaccttcacttctttaagtcgttaccgggcagggagtggcggggagttggatctgtcaca
gtgcccggctttggagggtggcagttttactggggtagagcctgagggacccctgtgggccctacaaccgcagagcccattcaggaggtttctccggaag
gacgtacagagcccaatacagctccagctttctctgtaccagggccatgaacctcttgggccactactggccactgccacccaggagaggggattcattg
gagaaggagtgagtcggataccggtgcgggagggcaaggtccggggcagcctcttcttgccaccaggagagggaccatttcctggtgttattgaattaca
aggaactggaggaggcctgctggaatacaaggctagccttctggctggtaaaggatttgccacacttgctttggcttactatggctatgaagatcttccc
aagcaaatgaaagattttcatttagaatactttgaagaagctgtgaatttcatgttgaaaaatccaaaggttaaaggtccagggataggacttctgggcc
attctaaaggtggagatcttgtcctctctatggctactttcctaaagggcataacagccacagctgttgttaatggttcccttgcaaatgtgggagctga
tttacattacaaagatatcatgctccctgctattggctttgaagcgaatagaattacatttccccagcctggagtggcagatatcagcaatgttctgaac
aatcctctcgaagaacctaaccgtgcaagcctaattcccgtgggcagggcaaacagcaagttcctctttattgtggggcaagaggacaagaactttaaaa
gtgatttttatgccaaaattgcttgtgacctattgacagagcaagggaaagaaaagccacaagttgtatgttaccctggagctggtcactacattgagcc
acctaattttccactatgcaaggcctctatgcatagactggtgggtcttccagtgatctggggaggagaaatgaaagcccatgccatggcacaagttgat
tcttggcaccggattcaggcctttctccatgagcacctagatacacaagataacatacccagcaagctgtaaccaagtaacatttggatatcccagttca
taccctgagactgacagcctacttttctgctctagaaaactaccattggaaaaggttgtctagtatgcaaagggcaagccccagaccagtttttgttgta
tccgttctatgcaaacctatgtccccaccttgatattttcttgtgtcatactgatatatattgaaatggtttaaataaatattaaataaaaaaaaaaaaa
aaaaa
5' EST: CX964743 [+]ccatcgattcgtttccataagctcctgttatttaaaggaaaatgctggcatctagattgaaagttatgttctgccttctgcgcaggcccctgcctagatt
cctgcctgcctgtgccatgtctgtgtctctgcaggtcaccccagggcactgcctgtatgatgagcccctacgggtggctgcttctggcataggacctggg
caggaagtgaccctccaggcaacagtgactgatgaaggtggggaaaccttcacttctttaagtcgttaccgggcagggagtggcggggagttggatctgt
cacagtgcccggctttggagggtggcagttttactggggtagagcctgagggacccctgtgggccctacaaccgcagagcccattcaggaggtttctccg
gaaggacgtacagagcccaatacagctccagctttctctgtaccagggccatgaacctcttgggccactactggccactgccacccaggagaggggattc
attggagaaggagtgagtcggataccggtgcgggagggcaaggtccggggcagcctcttcttgccaccaggagagggaccatttcctggtgttattgaat
tacaaggaactggaggaggcctgctggaatacaaggctagccttctggctggtaaaggatttgccacacttgctttggcttactatggctatgaagatct
tcccaagcaaatgaaagattttcatttagaatactttgaagaagctgtgaatttcatgttgaaaaatccaaaggttaaaggtccagggataggacttctg
ggccattctaaaggtggagatcttgtcctctctatggctactttcctaagggcataacagccacagctgttgttaatggttcccttgcaa
3' EST: CX964742 [+]atatttatttaaaccatttcaatatatatcagtatgacacaagaaaatatcaaggtggggacataggtttgcatagaacggatacaacaaaaactggtct
ggggcttgccctttgcatactagacaaccttttccaatggtagttttctagagcagaaaagtaggctgtcagtctcagggtatgaactgggatatccaaa
tgttacttggttacagcttgctgggtatgttatcttgtgtatctaggtgctcatggagaaaggcctgaatccggtgccaagaatcaacttgtgccatggc
atgggctttcatttctcctccccagatcactggaagacccaccagtctatgcatagaggccttgcatagtggaaaattaggtggctcaatgtagtgacca
gctccagggtaacatacaacttgtggcttttctttcccttgctctgtcaataggtcacaagcaattttggcataaaaatcacttttaaagttcttgtcct
cttgccccacaataaagaggaacttgctgtttgccctgcccacgggaattaggcttgcacggttaggttcttcgagaggattgttcagaacattgctgat
atctgccactccaggctggggaaatgtaattctattcgcttcaaagccaatagcagggagcatgatatctttgtaatgtaaatcagctcccacatttgca
agggaaccattaacaacagctgtggctgttatgccctttaggaaagtagccatagagaggacaagatctccacctttagaatggcccagaagtcctatcc
ctggacctttaacctttggatttttcacatgaaattcacagcttcttcaaagtattctaaatgaaaatctttcatttgctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: