XB-CLONE-3565663
Clone Name: IMAGE:7709677
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Gene:
slc7a7
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964747
[+]
ctcccgagtgggcgagagggatacaaaacatacgtcgcgtatttccattcatactgacgcactctcatccactgaagtgccttgacttggcacagacgca
tcctgccgcactttcattcgctgacgcatcgtcatctgcgttaaggcgcacttactaaagctgtctttaggacatggttctttgctgctgaccctcatat
tccaacatgggtacctctaccaagtacacacctgcctccacagaagagccaaaccgtaatgggacaaaagattccacagcagactcgggctcgcaagagt
ccatgaaactgaagaaagagatctccctgttaaatggggtgtgcctcattgtggggaacatgattggctctggaatctttgtgtcacccaaaggggtctt
aatatacagtgcttcatttggcctctcactggtattatgggcagttggtggtattttctctatctttggcgctttgtgttatgctgagcttgggactaca
atcaccaaatctggggccagctatgcctatatcctagaggccttcgggggttttgtggctttcatcagattatggacatctcttctcatcattgaaccca
caagccaagctgtcattgccatcacattttccaactatatgttgcagcctatcttcccttcttgtggatctccttatgctgctaatagactcattgcagc
aggttgtatagcactccttacctttgtgaaatgtgcctatgtaaaatgggggaccagggtccaggatatcttcacttatgccaaagtgctagctctgatt
gctgtcattatagctggaattgtaaagatctgtcaaggtc
3' EST: CX964746
[+]ttgttgtttttttctttctttcctgaataactgttatcaacacaactgctttattttagatggattaactgtttcatccaggagcacaaaaacttcaaaa
ggttttttccttccactggcatagccaaattccacaataaataactttgatttgaagggaaataagaaatcaatagtgtagatggctcccctttcgtgtt
ggattagccaggctcatattgctttacaacacaaggctggcccttagatcagaaagtagttaattccttgctttggaaataataaaaggtttaatattta
tgctattcaactattccctcccagagagaaatggtttgcccatgtccatccagagaactgctctgcagcaaccttgcaatagaaaggtagtttttttttc
ttttttaaaaaaggcacagtttatgcaataaattagtaaggcttcaggtttctttgagtcagccatggaatggtggacagttctatcaatcactttttga
gaagtctttttcctcttctgtatccatttcagttagtactgagtagcacagaatttgtgtatatcgtgtcagagcacctgcagcacgctgaagacattgt
ggcttccgtttctcagaaacacgaaccagaaggaaatagactgggactcctgacaatgcaattcccactccaatcagagaatttattagatcactataca
ggggtacagccaccaggaacacagtgcaaagacagaatatgattgggaacactaggctgagcttgagtggccttggtcggtctggttgtttccagcgcag
atacagctgtcccgcaatagaaagtcccacaaataaccagtaactgaaactgtagtaattaatgagctggaaaatatctttcacacagaggtacaccagg
gccattgcaccattaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: