XB-CLONE-3565597
Clone Name: IMAGE:7709683
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Gene:
slc13a2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964790 [+]
agaaatggaatttgcataaacgcattgccctaagagtgctgctgattgttggagttaaacctgccctcctgcttcttggattcatggtggtcacagcctt
cctttccatgtggatcagtaacacagccacaacggccatgatgatacctattgcacaggctgtgctgcagcagcttcacacctcggaagagagagtggac
aagccgtcagaagaaacaaaaaagaatgtgaatggaatggataatagcatgtatgaatcagtattgccaaatgggaaaatgatcctggcagtagaaaaca
cttatgctactgtaaacgagggatttgagatgcaagatagtactaaagatcctgagccttccaagctagaaaaagaaagtattggcccaatagtgataga
acttgaggatgaaaaacaaaaggaagagaagcagaaacttagattggagaagcacctgaagatctgcaaaggaatgacccttagtgtctgttattctgcc
agcattggtggaattgcaacattaactggcactaccccaaaccttgtaatgaaaggacaaatggacgagtaggctctttccagagaacaataatgtcatc
aattttgcctcctggtttggttttgcatttcctacaatgtttgtacttctggcattatcctggttatggctccagttcatgtttttgggattcaacttta
aaaagaattttgggtgcggtgggaattcagaacaaaaggaaaaggagaagagagcttttaaaatcatttctggngagcacaaaaagttgggcgccatgac
atttgctgaaatatctgtgctggtgctatttatacttctggtgctgctgtggtttaca
3' EST: CX964789 [+]acatacttaatgatgtatttgcattagtaacatgtagctcattgagctctgaaacctagggcacctagatatttcacccaatactgatagagccattaaa
atgtttacattatttttcttaaataaatacaagtgagatacttgccctgatacaaaaaattaagttcttagagagaactttttacactcttgcctgctgg
gtattgcccttattaacagtactattaactgataaggtccccaattaaaagctttgcagagcagagtgagatgctaccttgactattctgtaggccggta
gggtactcggcccatctgcttatagtcacagggtgggcacggcttgtgcgtattcacggacactagagagtctgaactgtcaatctgtggattctggcag
aagtcagtggggctgctgtaagatgccataaagaatcactgtttattgggctgatgggggactgtttgagtctcttggtagttgaaatgccagggtctat
tttgaattccagtctgaacctgtgccacatccaccactttatcatacttctgaggctccttggcatccagttaaacacaactggccattaacagtccatt
acaaatgctaaggctgccctgtggagtttgcccaggatggaaatgtgcccaagtcgaacatgtaaaatccccagctgttaatggccagtgttatagtcag
cacacccagaatgttgagcagtagtcctgcctttgccatgtcgataaccttgagctgcccataagagaaggcaatggcattgggtggcgttgcaactggc
agcatanaagccaaagaggccgatagtgtgcatggaagcatgatatacagtgggttcagatggattgctttggccatggatgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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