XB-CLONE-3565538
Clone Name: IMAGE:7709593
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Gene:
fsd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964830 [+]
atatgtttcgggggcacgccccttcgtcagacatcactggcgagggggtgtgcccccggggcgttattggtttgctgctattgggaaacagcggggtttt
tttctcttgaattgtgtcctgttgtgccggtatttttgtgttggttggattgtggtggagagtgccgaaatctctcctaatactatgcaccagggaaatg
caatacatatcaaaacattctccctattcttttatctctttgtacgaaattataaaaatagtatcagtttgagctacaaataatatagcccttgatatat
ctttgtattgtgcataatgtgtatttttttttgagcagcaatttatgaacagaacaatgaagatggagtggttacagtacgatatgcgcaggactcccca
ggaagcacaaaaacctccccacttcatactgcacctcctccccttccacgggatcctccaccagaagatgattatgagaacccagatcaaggcgagaaaa
gatggaattcatttcgagaagtatctaaagggccaagaactcccaaaccagttccaagctcaggcctggatgaagaactaagaagaaaattggaaatacg
aagacagcaaattaattgattgcttctggtttcatagaaaaaggactgaatattaaacactttaaactactaaagttgccagctgagaactctctggatc
agtgacttgcaaacataatgtgactcagcctttaaagcttcaaaaacagctccccttttaactgtaattgctttttgactctgcagtagaatttgaacct
gtgtactacagactttgccaagctggaggtctcttctt
3' EST: CX964829 [+]gtgtgtttagcttttagttttattaggagcaactgattaatatttacagttaggagaaactccttttgaagttatatgacaaacacattgaccagaaatg
taaccataaaatatcagatctaaatcttaacattaaactcatcctgaacacaaagactaggcaagctgtaccttaatagtatgatggctttagtacaata
tttgtattgttgctaattaccttctgtcaaccaacaggtatggtgtggaagtttctcagtgtatactggactccaggttcctagctgtgtgaaaagcctc
cagaaaaggaatagtgccaccagcagctcaagcaccagcctcacttgatttcatctgatctacccacctccttcatttccccacctagatctctcctttt
ctctccctttacaatgcattaaagcttaaagtccacccagtattttaggggacctattaaaaaaaacttgaacataatttagggttctacaacaaatatt
ccatgatatcctctgcacctgtaagagatggatggacagtacgctaagtgtggcattctgctgattatgccttaaataaatataagttacatgtagtatc
gcgatactgtcagcttgtttgtatgcataagctctatgtgtagcttctaaacatctataagctcatccccaagaactttctccatcttaggctgtttctc
cacctgctttttatttgttgtcatcagctcctttctgcatcagccatgtgtggaattagtctcagagccgaactatcttcactgcacatctgtaaatata
atgcattcactgcactggtggtgcaagccttccatgggactggcagtgtccct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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