XB-CLONE-3565437
Clone Name: IMAGE:7709460
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Gene:
cxcl14
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX964897 [+]
cacatccgaaggtaaactagcatcccaaaatcttctttcgagttgcctagatttgcaacagcggtagaatttctccccctttaatgcaaactcctctttt
cattgactttattagactgctgtatatgtaatctatcgtagggaaggccgcattttttattttttgagaccctgcctgtaaaatagcttatttggactcc
tttacaaagtgttacgctttagttgcagctaaaatggcttaatattctcaaaaatttggcactttgtggatataacgcactttgattttgtaaagattaa
ttccaaaatatgtttgtttttttaagacattacatttttttttatcttataaagtctggtaaaatgtttgccggattatttggcatgcacaatttattca
tagcaaatttggaaattttgtggtaaattatttggatgttcagtttctaatacagatttgcttctctattttgttagtatttattgatgcaaaataagct
tgtttaactcattcgtgcccacagcattagcaacaccaaaggctagattttgtaaagtggtcttttgctgaacccctttgagagtaagagaatgatagga
atgaatgaggaataaactcactaagtggccacttctgtctcaagtgccaagtgattataatgatgaaatcccccccccaaaaaaatgtaatttgtgatgc
tagacatatgatcaatagagatctgaatttattagcatctaaggttgcggtgacttggccattttctgataattgtaaataaattctctaattgactctg
aataccctatcagcataattgaaatccc
3' EST: CX964896 [+]catgcaactttctacgtaaggaaaatacataaatattacatgataatttagcaatacaatacattacatgtatagataatatacaaatatttatatgaag
cgtttctgttaaattttatttacaaattatagttattattgaatgatctgctttctgtgggaacttaagactgacacgtgtgtgtgttaccgtaaaagct
tgacgttctatagcaatgaattatgttcatttgcaaatctaaaatgggttgcacatctctgacagtttacattattattctattatttgtatatatgcac
acatacacatatatttaccccaaaaagtcccattaagttgacattcaaaaactttgtggcatgtccactgtccttaggatattttagataatgtaccatt
ataatagggttatgtaacaaacggtacaaagtctgcccaagagcattaacccatagtaactaatcagcagaaagcattacctgtataaaagcaaacatct
tatggttgctatgggttactgctgctgggcaaacttagctcctttttttgacacatgagggtaaatatcctaagcaggacgtataaaggcattttgtgaa
gttggctacgaacaccggcagccaaaatgtccaatataggacctcatctgacaatttgctaaatgattaaatttagaagatagctcagtattcatttgtc
ctttgattttaaatacgtgccactgacttcctgctgaatggatttactaatacgtcacccagaatctggtcaattccatgtagttgaccagagtatttgg
gtcttttggagttaagtaaacacagttgtgcagttttgttaaaaccttgccatcagtacctgtcatgtgtatccagattaagtcccatacgtaaattagt
aaccgacacatacagtaatcaatagtaactaaatagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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