XB-CLONE-3565156
Clone Name: IMAGE:7709534
|
Gene:
cel.2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX965094 [+]
cgatggcctgcctggtggtggcccatgccaaaacgctcggcgtagttcacaccgaagggggatttgttgaaggaaccaataagaagattggggttgtctt
ccccgattacattgatattttcaaagggatccctttcgctgctcctcccaagcccttcgaaaaagctgagaagcacccgggatggtcaggtaccctacag
gctaaagactacaagccgcgctgtttgcaagccaccatcacccaagacaatgtcttcggaagcctagattgtctctatctgaacgtctgggtgcctcaga
cccgctctcaagtgtccacaaatctccccgttatggtatggatcttcggaggggggtttttacttggcagcggtcaaggagccaacttcctgagcaacta
cctgtacgacggggaagaagttgccatgcgcggcaaggtcattgtagtgaccttcaactaccgcgtgggacccttgggatttctcagcactggcgactct
aatgctcctggtaactatgggctgtgggatcagcatatggccatcgcctgggtgaagaggaacattgcagcatttggcggggacccgaacaacatcaccc
tgtttggagagtcagctggaggtgctagcgtttcactccagacactgaccccgtacaacgtgggactgattaagcgggccatcagtcagagtggcgtggg
cctgtgcccctgggccattcagcgggaccctctcacttgggccaaatcgcttgcgtcaaagctcgggtgccctgtgaataataccaaagagctggcagat
tgcttgcgnaacacagacccaggcgccctcactatagcctatcagctgcaactgtttaacct
3' EST: CX965093 [+]aaagctgcaaaaataaattgtttaataagcagtcatttgaacctgttatgcatttggcaaatttataaacgtttgtgcccagaatctgacgtagggagaa
cgcaagctctgcttcatggactggtagtttatcttgtggttgatttccaggtattggccattctcagttgagtaggctagccagggggtaggaatgctgg
attcgcctttactggggtctccagtcgccgcaaagttggtccaatatgcaatcatgtactgggccacatctctgtctctgggtctgtaagccagcggggt
ggagaacggtttgccaaacataaactgcagatcttcggcgtggtcagcccccatccagctggggtaaacgggcatacggcttgggtggctgaacatatag
ctgtatgtcttggcgcccttggcattctggtagtgtagcgccagagcctcctgagtagccaccaggaagatatagtctgtttccacttccacgaccgtcc
tcttcatgtcttcttgactgggattggtcccccagccggctgtgtaaagatcgtacgcaatatcgagtgccggcacccctttggctagggttaagccccg
caccactttctggacctgttcggtgctgattttgtggagaggctggtttatagcaggcaaatcgacgccggcaaaaagatgtccatccatgttgtttacc
cccgctatgtagtcgacccccgctgcgttggagaataggttcctgggttcatcagggatgaagtctccatctatgaccggggagtaggccaagtaatgga
ctaaaggatattcaaggttaaacagttgcagctgataggctatagt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: