XB-CLONE-3564940
Clone Name: IMAGE:7709841
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Gene:
cpa1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965237 [+]
aattttccctagtgatgaggctcaggtgaacctggtgagggcgctggaagatgaggagtatttacagctcgatttttggacaagcccctcaagagttctt
ttacccattgatatcagagttccactccaccatctgcaatcagtgaaagtctacctggagtccaacagtatccagtacagcatcatgattcacgatgtac
agaacttgctggatgaagagcaacgcgatatgagacgtaatcgtgcctctgaggatggaaggtctactaacagcttcaattatgctgcctatcatacatt
tgatgagatcaacgcctttatcgacaacttggttagtgaaaatccaaatttggttagcaaaatacagatcggttcaagttatgaaggccgtcctcttaat
gttctgaagttcagcacaggtcccaaccgcccagctttctggattgacactggtatccattcccgtgaatgggtcactcaggccagtgctgtctggtttg
ccaaaaagattgtcacagattttggtaaagatccatccttgacaaatacactgaaccaaatggatatcctcatcgaaattgtgaccaaccctgatggctt
tgtttacactcataaatcggatcgtatgtggcgtanaaccagatctccaaattctggctcactctgtgttggaactgaccccaacagaaactgggatgca
ggttttggaggtcctggctctagctcanatccctgcactgaaacctacagaggacgtgctgctcattctgaacctgaagtgaaagccattgtggactttg
tgaaaagccatggcaacatcaaaggctttgtctccattcacagctactcccagatgctttctcttcccatacggatacactaatacccg
3' EST: CX965236 [+]caaaaggtgtaaatgttgtttaataaaggttatcacgggtatgctcaataagtatagtgagtgcaagccatgtctcctctgcagttggaatgatttggtt
agctggtagtgcaaatccatagcgcccagtatcacgcagctcaaagctgtaggaatgcttaattccttgattgtaggtccagtcaatggttcccccgctt
gcttggtagatggtactgatgatgctgccataggtgtaagctgtaccgtacagtgaagccaaggcagtaatagctttctttgacacagcattcagctcat
cgtggtcgggtactcgggtattagtgtatccgtatgggaagagaagcatctgggagtagctgtgaatggagacaaagcctttgatgttgccatggctttt
cacaaagtccacaatggctttcacttcaggttcagaatgagcagcacgtcctctgtaggtttcagtgcagggatttgagctagagccaggacctccaaaa
cctgcatcccagtttctgttggggtcagttccaacacagagtgagccagaatttggagatctggttttacgccacatacgatccgatttatgagtgtaaa
caaagccatcagggttggtcacaatttcgatgaggatatccatttggttcagtgtatttgtcaaggatggatctttaccaaaatctgtgacaatcttttt
ggcaaaccagacagcactggcctgagtgacccattcacgggaatggataccagtgtcaatccagaaagctgggcggttgggacctgtgctgaacttcaga
acattaagaggacggccttcataacttgaaccgatctgtattttgct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: