XB-CLONE-3564892
Clone Name: IMAGE:7709941
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Gene:
actb
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965270 [+]
caaagccggctttgcaggtgatgatgcaccccgtgctgttttcccatccattgttggtcgcccaagacatcagggtgtcatggttggaatgggacagaaa
gatagctacgtaggagacgaagctcaaagcaaaagaggtattcttacactcaaatatcctattgagcatggcattgtcactaactgggatgatatggaga
agatctggcatcataccttctacaatgaactcagagtggccccagaggaacacccagtgctgctgacagaagcacccttgaatcccaaagctaaccggga
gaaaatgacgcagatcatgtttgagacctttaacactccagctatgtatgttgccatccaagctgtgttgtccctgtatgcatctggtcgtaccactggt
attgtcatggactcaggtgatggtgtaacacacactgtgccaatatatgaaggttatgctctaccccatgccattctgcgtctggacttggctggacgtg
acctgacagactacctcatgaaaatcctcactgagagggggtacagcttcaccaccacagctgaaagagaaattgttcgtgatatcaaggagaaattgtg
ctatgttgctctggactttgagcaggagatggccacagctgcttcttcttcatcattggaaaagagttatgagctgcctgatggacaagttatcaccatt
ggtaatgagcggtttagatgtccagaagcactcttccagccttctttccttggtatggaatcctgtggcattcatgaaaccacttacaactcaattatga
agtgtgatgtagatatccgtaaggacctctatgccaatactggtctctctggtggaaccacaatgta
3' EST: CX965269 [+]agtgagaaacggatgtgaaacaacttggttttatttttttcagttaggttacaggtgacccgtcacatcttatgtgatcaatgataatgtaaaccttcca
ggtgttagagaatgatgcccttttttgggttaaacaagttacaagtgttcaaggcagaagatacagagacatgtttgggtacatagctgcacaagaaaaa
aaaaacaaaaaaaaaaaatcaaaaaacacaatagtatcaccatgatttctgtatgagcttcccaccaagctgggcaacattgaaagggtagcttcctcaa
tacaatactattgactgaataaaatcataatgtcctctgcattgatgaccatacagtgctatttgttcattccagttatcaaatctgagccaagcgccaa
tgaaagaaaagccatgccaatgttatcttcctgcacaggtacatttgttcacgttgaaagggtccgtccttttagaagcatttacgatgcactattgagg
gcccagactcatcatactcctgcttgctgatccacatctgctggaaggtggacagggaagccaagatggaaccgccaatccagacagagtatttacgctc
agggggagcaattatcttgattttcatggtgctgggagctagtgcagttatttctttctgcattctatcagcaattccagggtacattgtggttccacca
gagagaacagtattggcatagaggtccttacggatatctacatcacacttcataattgagttgtaagtggtttcatgaatgccacaggattccataccag
gga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: