XB-CLONE-3564863
Clone Name: IMAGE:7710039
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Gene:
ptgr1.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965289 [+]
aaagtggtttcatcatcttggataaacatgttatttaaatattattaggaggcgttgtgaaagcatgatccatttcccacagtgcacccacagtgcaaca
ttcttgctcacgtcccttatttagcagtaattatggctttcccagtgttatctcctttaagcattccaatgaaagcagatggcaggttgtcaaacccttt
ggtgatgtgctcacgatacttcagcttcccctcaacaacccactgcatcagcttttccaggccttcgttgtatcggttctgccagcgtggcacggtgaat
ccttccatgcgtaactcttgtaagattaaagggtagtggacatatggtcctgtagagaaagttaaacattttttagtgattttaaactgcaagtgactac
aaggtcccatgaaatactggaacccgttatccagaaagatccaaattacggaaaggccatctcctatagactccattataagcaaataattctaattttt
aaaaattatttccgttttctctgtaattataaaacagtaccttgtacttgatcccaactgagatataattcatccgtattggaggcaaaacaatcctatt
gggtttattcaatatttaaatgattttttttatcagacttaaggtgtggcgatccaaattacagaaagatcccttatctggagaaccccaggtcccatac
attctggataacaggtcccatacctgtacaatcatttttaaccatccttgtgctggaaaaattaaattgttatcgcttagggacttgngaatatgttttg
tgcagctaaaacaccatctaaacaaaactggcattaacaactgtctctaagggtctatttatatatccagtccca
3' EST: CX965288 [+]ctagaatggaacccggtctattcgtacattttgaaacatagataaaaattctagtatcttgatgggatacaaattaggggtcagttataaaggagtatgt
acaggcaaaacggatgtatcttttccttcaaaaaaatagattagcaaaaataattaaaggaatactttcttatgaaaatccatagaaatgtgtaaatact
actaaccagttcggttaggttaaagtactctacagccaccctctagcccagcggtaactgctcatgtcgtgcaaggggactgagagcaaaaggagccaca
ctgttttcctgtctcacactgtgttgggggcaatgcaatccttccatagcctaattacacatggacacagtactccagcctatggaaggattgatctgcc
tttagcacagagtgattgacacaactgcagaggatcctcttcatgttttggagtgtcgttgggtaagtaccactcagctgggggtgcctttgggggactt
ttcgctagctacatttttatgctgcagccctttgttgctgactcaattctagggattttctcatgaatgtgtcagtgtccctttaagtgaggcatggtgg
gatacaatcaccaaatgtcccctgcactgttatatcagaaatgtgacaaaaaaaaaatacaacagtttcaccaatctttggttttcaggtcagatataca
caccaaaagctctcgctgtgtctcaggtacttctttcacacaaagcgactggccggctagtttttaaaaaatcctgattaggcagcttactctgttaaaa
gtgaatgagcactaagggacacaagagtgcacccataagtgctcatacagagggcacttttgcccaggggtctggctgctttgtggacctcatgcaaatg
aaaaaaaatcagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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