XB-CLONE-3564825
Clone Name: IMAGE:7709899
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Gene:
ocln
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965314 [+]
ttgtccataaaggagttatacaaactgtcaattagatgctacattcattaagcttttttagatacactgccgacattgcacaccttattatacaaaatcc
taccctaactattcaaactgacatatataaaatatcatgtttataaaaactgttttgcacactgccttattgccacttacatgttacaatgcagttagtt
atgttatctgaattaatgttagcttctatcacactggtaattgcacttgtcaaaaaactgagtattaaaagaaaaggcactgcatggctaccaccttact
gatacataagtaaaaaaacggcaaaagtctgctcttagcacatttgcacacagtaatctccaaagcggttcacgctaaaagggtgataaattggcagagg
ctggttataatcacagttctcatataatacatttgttttgcagtcacatgaattggctcattttaggcattctaagcatcattttgttctgcaaatagtg
aaaccacaataagcctagcccaaggttttggacattgcccagttttaacacattttgattagctgtgaggttacctacactgaaaaacgccacatttaaa
atacatcttaaaattcttcctatcctgaggcctatgcctcataaatgacaagaaaggatatgatcatgcatggctttcctcaagagaagtcagcctgatt
ttttccaactacttaaatatagctttcagagtccttcaagaaaaaaaattcagcaggttaggggacacgtttattttacagcaaggaagtggacaagcta
tatgcaacctatttagaactatatgtagatgggtcagatttacaaaagcactcacatacttttaagtaggctaaa
3' EST: CX965313 [+]tggagccctagtcttcattgcncaccattgtttataccataggagtaaatcctgtagctcaggcttctggatcagcattctacacccagattgtttcaat
atgcaaccagttctattccccagtacaaaccggaatatttgtgaatcaatatctttatcactactgtgtggtagagcctcaagaggcaattgcaatagtc
ctggggttcttgattgttgtggcttttgccataattatcttcttttctgtaaaaatacgaagaaggatcaaccagtatggcaaagcaaacatcctgtgga
agaaaaatgtcatgtatgaagatggagatcctcaagttgaacaatgggtaaagaatgtagctgaaaattccgcacctcccttttctgattacaatgaaaa
agtgaatggctctgtagcggactacagaagtgtaaatggggttcaagcctacccaccacagaatatctcacatccgataccagaggaagagttgcctttg
aaagaagactatggcatgtccccacatgcctatagcagtagctccgataccacaaccaagaaagcacctcctaagaaacggccagcaaaacccaggcggt
ctgacattgatacccatgaagcaggctataatacaggaggagagtctgcagacgagttggaagatgatagctgggacagtgagtacccacccatcacttc
agaccagcaacgacagcagtacaagcaggagtttgccagtgatctgcaggaatacaaagggctgcaagcagaacttgatgagatttcaaaatccctgtcc
cagatggataaagaactggacaaccatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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