XB-CLONE-3564685
Clone Name: IMAGE:7709913
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Gene:
slc34a3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965409 [+]
ctgtcctgaaggggcagattgctcaggcaattaagaagatcatcaatgcagattttccgttcccgtttacatggctaagtggatatctggccattattgt
tggtgcggtaatgacctttgtggtgcaaagtagctctgtgttcactgctgcgatcgttccattgatcggcgtgggggtcattagcatggagagagcctac
ccgttgttcctggggtccaacattggtaccaccaccaccgcagtgttggcggctctagccagtcctgccgatgccctcagcaattccgtgcaggttgcct
tcatccacctgttcttcaacctcacgggattggttctctggtacgtcgtaccattcctgcgcctccccattcctgtagccaagatattcggagacatcac
agccaaataccgctggtttgctgtcctctatcttctcctcagtttcctcatcataccgctggcaatcttcggcctttctatggctggctggattgtgttg
gctgctgtcggaggtcccatcctctttgtggtcattgtggtcatcattgtgaatgttctgcagaagaaggtccccaagtgcttgccgccattcctgagag
actggggcttcttgccgacctggatgcactccctggcccctctggacagattatttagctctgtctgcggctgctgttgcaaaaagtgtaacaagagcga
ggaagagaagccaaaggagcaactccctgcagacctgtcgttgggtgacatgcactgctatgacaatcctgattttgttcagtcacagagtctatgaggg
tgacaccagtcacctaca
3' EST: CX965408 [+]tttttttagctttaatttcctttaatgcagtcatattgtgccatttatttcacaggcgctgtaagcaaaagaaccaaacgtgacaggatcttccccaagg
ataaagggcacggctaaaaccctgtcgtctgcagctcactgctatgatttcctcttatgtttgggggcagaagcatgtgtttgcaggtgaaatacctata
tgtgatttattatatatgctcttatattaccatggggcggagcttctcccatacgcccatacaagtcgctgtaagtcgcttagccctgaacagaaatctc
attatcagttgttggggggtataaaaggggcaaaaaaaaccctagcaccatctttgtaaaacggtaagttttcctctgtaaaatagccactggctagagc
tcctctccatcctgcctggaatcacattccgagacgacccacctgagctacagataaacccatacgacttccactactggcatctgggctgctctcttcc
cagtgttcaaacaggaggacgttgatgtctgaggaccagagagaacagtgtatggttgcccagtggaggttttttataattacagagatgtccagcttcg
ttctctggtcttcggtggaaaatattccatgtgtcctttacgtcacaaccacagggaatagagcagccaatcagcaggacgtaaggaggaccacaagccc
atacaggtaggataataacaagtttggacatcctacttggactccagtacccaaaggcaggatctgaatacccctttcaagctggtagtga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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