XB-CLONE-3564493
Clone Name: IMAGE:7710028
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Gene:
tsc22d3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965545
[+]
ttattgcagctttccactgaattatgtacaatccagaaatctcagacagtaatgttaaacagcagaaaacattattcgtccaacaagcccttttatggcc
attgctgggagctgtagtcctgaagtttttaggaatattgcaaaaaaagtccacagtctattaattttcccggattttaaagtgtcttgtgtgagtcttt
caggcggcttttcccaccattgtgctctggcatagagtcagacaggtacatttatattctacacatacataaaaagacaaagtaaaaaaaaaaaaaagtc
tgtgtagcatattcccaagttctcaaagagaacattttggcaagtgctaaaattacatactagctatgtaaacttaaactaacaagcccagtagtgggtg
gggtctaaagagactgccaccgatcactagtgtgggcaactgtgagccaacgtataacaatctccattataaagcactaaagtgccctatgtattatact
ttgtactgaactgttactagggtaactgttccttatcctcattccaggaggttaacgctctctgcttcccactatggtacagtttataaaaacacacaga
gagggagatctcctcatcaactttgtattccatgcccagagaactcttgcagaagcagtctctggtcatctttagcatgtcctgcttgtttcctggcttt
gcttcccaagcctggcgccaaagaaaggcaacattcatgccgagccagctctctctggctggtactcttcttaaagagtccatgtctagcttctctcact
cttctcttga
3' EST: CX965544
[+]ctatagggcgagaggctaggagtcccataaaggacaggatatacgttttactggagctctatatattgaggagccgccgtgccaaaaacatgagcacaga
aatgtgtaacttcccaatggaggtggccgtgtatgagctgcacaatttctccatctcgtttttctcctccttactgggggctgatgtggtgtccgtaaaa
ctggataacagtgcatcgggtgccagtgtcgttgccatcgacaacaagattgagcaagccatggatttggtaaagaaccatcttatgtacgctgtgcgtg
aagaggttgaggtgctgaaggaacagattaaagagctggtggagaagaactcgcagcttgagaaagagaatcatctactaaagaacctggcaagcccaga
gcaaatgaagaagttccagtctcggctccctcaagatgaaactccaatcaagacaaaactagatttccttcgcgcatctcagaaggctggctctgcagtc
taagtggctctgtctttggagtgggcagagccactgaacttttcctgcgtctatatatgagaaaaaaatgagatttttttcccccctctttaaagtgtca
cctcggcttggagggtcgttgactagccaaagctggcatgtgccggggctagcgtgggcctcctcacaagtcttttcttcaagagaagagtgagagaagc
tagacatggactctttaagaagagtaccagccagagagagctggctcggcatgaatgttgcctttctttggcgccaggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: