XB-CLONE-3564478
Clone Name: IMAGE:7709886
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965555 [+]
cttacgagaaagcaacctaaatgacaatgcctacagacaaagcaacggactgcatcctgggagccccaaagtcacacgtaaaagaataagaacataaagg
aggtttctgagttcagaatcttcaaactgttgctgccttcctattctaacaactctaacaaatgaagatgcttggtctagtctggcgaagggttgttaaa
tttgatttgccaatgaattttaggggtttatgtggtgcatcctttttatttttttttattctgtatttgcagtttcagtatatcatattggaatggaatc
ttttcccatgttttaatggcttagatttaagatgcagtttaaaagacctcagcatgctgaatgtactgagcatgctatgtacaagaaaattttaagccgt
ttccaactgtttagatacaccaagattttagctcttgctcaaataagtaacccttttccactcgctacattattttactttttattaggggtccctacag
atctgatgtagcagcctgaaatccctgtgtacatgattcctatatcccttttacttgaatatgagccccatagaatagcataagggggcattttctcact
gcctatggtattttcttttttgtcaacatacaaggtgctataatgttttatattttaaaatgagaagctacatgggggatcagcagttttcttaaaacta
ggtaaacatgcgtatgcttcttttcttaagtgtcagaacatttcagatgtataacagcacttgacaagaatgactatganagagtgccagaagagcagat
atccataatcacattataccagatcctactgcttggctaaatcatgccatgcatatctacagtatatcatttgttctctataaactagatt
3' EST: CX965554 [+]tgatttctgttgtctaatatttaaaaactatgactgaaacactcccaatctgtgactttcagtttcaccaatttcctatgccaaacactcaaatatctag
atttaatggtcaagaacaacaaaatccaaataactgcaagactggataaaaacatctaaaacacacagaaacaaatctagtttatttgactagatgtgca
gaatttaatgaataagcttttatttcttattatgttttgaatctagtttatagagaacaaatgatatactgtagatatgcatggcatggatttagccaag
cagtaggatctggtataatgtgattatggatatctgctcttctggcactctttcatagtcattcttgtcaagtgctgttatacatctgaaatgttctgac
actttaagaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcccccatgtagcttctcattttaaaatataaaacattatagcacct
tgtatgttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgctattctatggggctcatattcaagtaaaagggatataggaatcat
gtacacagggatttcaggctgctacatcagatctgtagggacccctaataaaaagtaaaataatgtagcgagtggaaaagggttacttatttgagcaaga
gctaanatcttggtgtatctaaacagttggaaacggcttanaattttcttgtacatagcatgctcagtacattcagcatgctgaggtcttttaaactgca
tctntaatctaagcccattaaacat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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