XB-CLONE-3564472
Clone Name: IMAGE:7709982
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Gene:
scmh1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965559 [+]
caactgcttattgtctgtcagtggagtctccagcttatctgttgaatcttcagagttttctcaacctgggaccatgcgaaaacctggacacctgagagca
accgactggaaggatggaaggagacatggacgaccatcagacactcacactaatcagggtcacttcagttgggagaaatacctaaaagaaactgggtccc
agccagcaccccctcactgcttccggcagtcatgttcacctccctccaacgattataagataggaatgaagttggaggcccaagatccaagaaacacaac
atccacctgcattgcctctgtagtgggtctcacaggagccaggcttagactgcgactcgatggcagtgacaacaagaacgacttctggagactggtggac
tcttctgagatccaacctattggaacatgtgaaaagggtgggggcatgttacagcccccactaggcttcagactcaatgcctcctcgtggcccatgttcc
ttctgaagactctaaatggagctgaaatggcacctgccaaaatattccacaaggagccaccatcaccaccccagaattattttgaaattgggatgaaatt
ggaggcagtggacagaaagaatcctcattttatctgccctgctaccataggagaagtgagaggcagtgagattcttgtgacatttgacggctggagagga
gcatttgattactggtgtcgatatgactcccgggatatatttcctgtgggatggtgctcccttactgnggataacttgcagcctccagggaccaaagtgg
ccattc
3' EST: CX965558 [+]agggcgagaggctttttgccatcccgcagaaaacattttattgataactggcttctggatagtgagacctgttaataaaactggccccattataacccca
ataaaccattagattacaaattcttttgggtaatatttcattgtgcctaatttattctactatgtacagcgctgtgtgcactgtcagtgctatataacat
aacaaaagtattggttgtattcagcacacaacgaggtgcaggataatcctacatcccttcaaaaatgtaatgccttgtttccccctcttcccactagtgt
gaaatattaaggacaagttcataggtttagaggaaccactcttctattagaaactgtttttatcagccaaataaaatcccccaaaatagggattttcggc
atgttgcatcatgttccttgaagagaggttctccagaagcctgccaaggcagaagagtcctaggggtcagtatataaagggtgcaaagacgagccagaag
tagtggtcagaacttcccttgcttgagtttgtctatgtggtgagagagctttagggccggacctagcttcagacccatgtatttcatcataacatcactt
cttaagaggagcaaggccttgccatcaatttcatgtttacggaaaagctcagcatggaatccgagctgtgggtcagcatctctgacaaactgcatgacat
cctctgtgttccacaaggatgggtcacgtggtgccggccggccacttgagtacctctctgactcagctgagctcagccgtcgcacagttccctgggaaat
actgctaggttggtggacgctacgataagagggagagcgatactccctagatactcggctagtgttggggcta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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