XB-CLONE-3564463
Clone Name: IMAGE:7710174
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Gene:
naa15
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC167370 [+]
ttctgacaggcctgggcataggaaggtccggaaggagactggctcgtcccttctattgtgctgcgcatactgcagccgggcacttgcgtgtgttgtaggg
tagtgactgctcatccttatctctcccagcggccctcaggcagccgaaccacggcccgcgaactgtgcccataagaagggagcgggaggtgccagggaag
agctgctcctgcagtggctgcgacgatgccgagcgtcagtctcccgcctaaagagaatgcactcttcaagcgcatactgagatgttatgaacacaagcaa
tatcgaaatgggttaaagttttgcaaacagatcctctccaatcccaaatttgcagaacatggagaaaccctggcaatgaaaggattgactttaaactgcc
ttgggaagaaagaggatgcctatgaccttgttcgcaggggcctgaggaatgatctgaagagccatgtctgctggcatgtgtacggtctcttgcaaagatc
agacaagaagtacgatgaagccattaagtgttacaggaatgcactgaaatgggataaggacaatctccagatcttgagagacctctccttgctgcagatt
cagatgagggacttggaaggttacagggaaaccaggtaccagttactgcagctaaggcctgcacagagagcctcttggattggatatgcgattgcttacc
atttgcttgaagattatgaaatggctgcaaaaatactggaggagtttcgtaaaacccagcagacttcaccagacaaagtggactatgaatacagtgagct
acttctttatcagaatcaagttctcagagaggcagagctctataaagaagctctggatcatctttgcacctatgaaaagcaaatctgtgataagctagct
gttgaagaaacaaaaggagatttgctgctacgtttaggacggcttgatgaggctgcggaagtgtacagtgcattgcaggaccgatgccctgaaaactggg
catattacaagggtttagaaaaggcacttaaaccagaaagtatggaagaaagactgaagatttatgaagacagctggacaaaatatcccagaggattagt
gccaaggagactgcctttaaacttcttagcaggtctaaaatttagagagtgtctggataaatatttgaggatgaattttagcaaaggatgccctccagta
tttaatactttgagatcattatacagcgataaagaaaaggtagagattattgaagatttagttgttggttatgaaacatccttgaaaagctgccgtttat
ttaacatcaatgatgatgggaaagaagagccaccaacaactttactgtgggtacagtactacttggcacaacactatgacaaaattggccagccatcaat
tgctctggaatatattaacgctgcaatcgacagcacaccaactcttattgaactgttccttgtcaaagctaaaatttataagcatgctggaaatataaaa
gaagctgtccgatggatggatgaggcacaggcactggatacagctgaccgatttatcaattctaaatgtgcaaaatatatgttaaaagcaaacctaatta
aagaggctgaagaaatgtgctcaaaatttacaagggaaggaacgtcagctgtggagaatctaaatgagatgcaatgcatgtggttccaaacagaatgtgc
acaagcttacaagtccatgaataaatatggcgaagcacttaaaaaatgccatgaaattgaaagacattttgtagaaattacagatgaccagtttgatttc
cacacttactgtatgagaaaaattacactcaggtcatatgtggacttattaaaattagaggatgtactaaggcagcatccattttacttcaaggctgcac
ggattgcgatagaaatttatctgaaacttcatgataatcctttaacagatgagaataaggaacatgaggctgacacagccaacatgtctgacaaagagct
aaagaaactacgtaacaaacaacggagagcgcaaaagaaagcacaactggaagaggagaaaaaaaatgcagaaaaagaaaagcagcagagaaaccaaaag
aagaaaaaggaagatgatgatgaagagattggaggaccgaaggaagagcttataccagaaaagttagctaaggttgacaatcctttggaagaagctatta
aatttttaacacctcttaagaacttggtaaagaacaaaatagaaacacatctttatgcctttgaaatctacttcaggaaagaaaagtttcttttgatgct
gcagtctgtgaagcgagcatatgcaatagatccaaaccatccttggcttcatcaatgtcttgtccgattcttttgtgcagtttctgaaagtaaagaacta
aacgaatccgttcgaacagtgttaaagcaagaaatgtgcagactttttggagaaacaagcccagcaaattttaataattccttcctgaaaaaacatacca
actctataccacaccgattagcagctgccaaaatgatgtactatctggaccgttcaagtcaaaaacgagcagtagagctggggacaggattggatgaatc
gctcagtaaccaaagtctccagacgtgcactgatgttctggaaggcttgcgagacggcagtttaggagaaaaggaggctgcagaatgttatcgagtcagt
tgccacaagctctacccttacgctttagctttcatgcctcctggttacgaagaagacatgagtatcacaattaatggagatagctctgctgaaacagaag
aactggctaatgaaatgtgaatagctgataaagaacagacattggaccatatcaactatctaatatttttgtcacgcacctgctgtatcgctctgtaact
tatgtggagaatgaaaggagtaaaagaattgttgcctttaaaaaaaa
5' EST: CX965565 [+]tgggcataggaaggtccggaaggagactggctcgtcccttctattgtgctgcgcatactgcagccgggcacttgcgtgtgttgtagggtagtgactgctc
atccttatctctcccagcggccctcaggcagccgaaccacggcccgcgaactgtgcccataagaagggagcgggaggtgccagggaagagctgctcctgc
agtggctgcgacgatgccgagcgtcagtctcccgcctaaagagaatgcactcttcaagcgcatactgagatgttatgaacacaagcaatatcgaaatggg
ttaaagttttgcaaacagatcctctccaatcccaaatttgcagaacatggagaaaccctggcaatgaaaggattgactttaaactgccttgggaagaaag
aggatgcctatgaccttgttcgcaggggcctgaggaatgatctgaagagccatgtctgctggcatgtgtacggtctcttgcaaagatcagacaagaagta
cgatgaagccattaagtgttacaggaatgcactgaaatgggataaggacaatctccagatcttgagagacctctccttgctgcagattcagatgagggac
ttggaaggttacagggaaaccaggtaccagttactgcagctaaggcctgcacagagagcctcttggattggatatgcgattgcttaccatttgcttgaag
attatgaaatggctgcaaaaatactggaggagtttcgtaaaacccagcagacttcaccagacaaagtggactatgaatacagtgagctacttctttatca
gaatcaagttctc
3' EST: CX965564 [+]ttttttttaaggcaacaattcttttactcctttcattctccacataagttacagagcgatacagcaggtgcgtgacaaaaatattagatagttgatatgg
tccaatgtctgttctttatcagctattcacatttcattagccagttcttctgtttcagcagagctatctccattaattgtgatactcatgtcttcttcgt
aaccaggaggcatgaaagctaaagcgtaagggtagagcttgtggcaactgactcgataacattctgcagcctccttttctcctaaactgccgtctcgcaa
gccttccagaacatcagtgcacgtctggagactttggttactgagcgattcatccaatcctgtccccagctctactgctcgtttttgacttgaacggtcc
agatagtacatcattttggcagctgctaatcggtgtggtatagagttggtatgttttttcaggaaggaattattaaaatttgctgggcttgtttctccaa
aaagtctgcacatttcttgctttaacactgttcgaacggattcgtttagttctttactttcagaaactgcacaaaagaatcggacaagacattgatgaag
ccaaggatggtttggatctattgcatatgctcgcttcacagactgcagcatcaaaagaaacttttctttcctgaagtagatttcaaaggcataaagatgt
gtttctattttgttctttaccaagttcttaagaggtgttaaaaatttaatagcttcttccaaaggattgtcaaccttagctaacttttctggtataagct
cttccttcggtccctccatctctcatcatcatcttcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: