XB-CLONE-3564460
Clone Name: IMAGE:7709840
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Gene:
ddx21
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965567 [+]
aatcatcgatgcccgtgaaggtttatgtcgatgaaatggaggttgaaagtgtcaagaagaagaaacagtctgaaactcctctacccaagataaaaaagag
aaaaatgcagaatggggcaacagaagatcttgatttggaacaggtggcagaatctgtaaatggagagatcaacaacaataatccgactcccaagctgaag
aaaaagaagaagcctgtgccacaatcagatctctctgaaaccgcagaacagtatgaaggagagcagccagatccaagcactcccataccaaagaaagtca
agaagaaaaaactaaaagagggcaaggaagattcagacacacaggaagggacagacaaatccgaaccacaaatgaacggcgtaaagagtgttaaaaaatc
taaaaagaacacaacaggtgatgataatgaaccaactcctaagaaaagaaaaaaggatacaatggagatcacagcatccgaggagtgtgaagaaaagcag
ttaaccaaagaggaagaggaaataaatcaagaaaaaatagatggcgacttctctaaatttccaatttccaaggacaccattaagaatttgcaagctaaag
gcgtgtcctacctgttcccaattcaatccaaaacattccacactgcttacagtggcaaagatgtagtggtccaggcccgtacaggcacagggaaaacatt
ttcctttgctattcctttagtggagcggcttaatgaggaccaacagccgttggctagaggacgtgcgcctagggtgataattcttactcccaccagagag
ttagctattcagatcacaaatgaaatccgcagcatcactaaaaagctgaaggtctgttgtttctac
3' EST: CX965566 [+]ctaagcacacagttttatattgcataaaaatatgtctcaagttactttaggaaggaatatttatgaacatggcagtatattaaaaaaagttattgtgaca
tgggatgttgggatgtttggagtcactaacggccccttctaaaccctccacttctatttctgcctcttcctcctccctcttcctccaaaacctccattcc
ttggaccatcaaagtttctctcagattcttgaatcgcgggtaattcagttgcaactgtaaactgccaccgccttgtatctttccagccctcctgcattga
ctgcaggttttcagaacggacatcaaaacacacacccatggaatccttcaggagacacattctatgaattttagaatcaatatcttcacccagttgttct
ttaattgatcgccatgcatagcttaagttgtgaatttgcactgagctcttcaacgttattgtcacataacccgcttccatgttgagcaaagagcgttgtt
taatggacgtcgccccagaaatatgagctaaagctgcagctagggcagttaatgcacccttcttctcaatcagctcctgggcgtattctttaaaatgctc
aatcacatcagctggaactgaatccaaggactttattgcatcagcactggatgatttggcaacattcaataaggaaggtattccaacacgtttgaatgtg
attcccgtgctcctctccacattccgaagataatgcctttctcttggttcataaagggatatgcagattcctgtgcgtccggctcttcctgtgcgccctg
accgatgaacataagcatctgcttcctttggtgcagagtaccatactacaaggtcaacttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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