XB-CLONE-3564374
Clone Name: IMAGE:7710182
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Gene:
f7
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157198 [+]
CCTTGGCGGATTTATAACAGAAAGAGATTTCCTGCAACATGGATCCAGGGCACAAAAAAGCTTTCTGCTTCTGTTTTCTTATGGTCCTTTCATACATTCC
AACCTTTGCAGAAGATGCCAGTCTGGCTCAGTTGCAGGATGCTGCTGGTGGATATAGAGAGTCTGCAGATGTTTTCTTACAGGACAAAAGGGCCCATAGT
CTGTTGAACAGCAGAAAAAGAAGAGCCAATAGCTTTTTTGAAGAATTTAAAGCAGGATCACTTGAACGAGAATGTATTGAAGAGATCTGTTCCTATGAAG
AGGCCAGGGAAATCTTTCAGGATGACAGACGCACAAAAGAATATTGGAAAGTATACACAGATGGAGATCAGTGTCTCTCCAACCCGTGCATGAATGGTGG
AACCTGCTTTGACCAGCACCAGTCATATATCTGTACCTGTCCCATGGGCTATGAGGGTCGGCACTGTGAAACAAACTTGAGGGACATGCTGAAATGCATT
TATGACAATGGGCAATGTGAACATTTTTGCCATGACAACTCATCGACAAGCCGCCAGTGCTCCTGTGCTGAAGGCTATAAACTGGGAGCTGATGGTCTAT
CGTGTGAACCTACAGTTAATTATCCTTGTGGCAAAATTCCAGTACTGAAAAATGTAAATAAACGTGCACGGATTGTGGGAGGTGATATGTGTCCAAAAGG
TGAATGCCCCTGGCAGGCTCTTCTGATGTATAACGAAATATTCATTTGTGGTGGAACTTTAATTGCCCCAAACTGGGTAATCACAGCAGCTCACTGTTTA
AAGCCACTTCCAGAAAACAAACTTACGGTAGTGCTAGGAGAACATAGAATTGGCACACCAGAGGGAACTGAGCAAGAGAGCAAAGTTTCTAAAATAATAA
TGCATGAACATTATTATGGGTCTAAAACAAATAATGACAATGATATAGCCCTCCTTAAACTGACTACGCCTGTCAACTATACAGACTATGTGGTCCCTTT
ATGTTTGCCTGAGAAACAGTTTGCTGTACAAGAGCTCTTATCGATTAGATACTCTACAGTCAGTGGCTGGGGACGCCTGCTTGAGAGTGGGGCAACTCCT
GAACTACTGCAAAGAGTACAGCTGCCCCGAGTCAAGACACAGGACTGCATCAGACAGACTCAAATGAATATTTCACAAAACATGTTCTGTGCAGGATACA
CTGATGGCAGCAAGGACTCTTGCAAAGGGGACAGTGGGGGGCCACATGCTACACAGTACAAGAATACCCATTTCCTTACTGGTATTGTCAGTTGGGGCCT
AGGATGTGCTAAAAAAGAGAAATATGGTGTGTACACCAGGGTATCCAGGTACACTGAATGGATAAAGGAAAACATGGATGAGCAACCAGAGGCTTAAATA
AGTATGACACAAAACTCTTGCATAAGAACAAAAGCCTTCAACTGATGTCCTTTGCAAAGCTACATCTCCTATGCAAGATTAATATGATATGATCACAATG
CCTTTAGTACAGTCAATCACTGACATAAATAAAGAATAGGAAAGCTCACCCAA
5' EST: CX965624 [+]ccttggcggatttataacagaaagagatttcctgcaacatggatccagggcacaaaaaagctttctgcttctgttttcttatggtcctttcatacattcc
aacctttgcagaagatgccagtctggctcagttgcaggatgctgctggtggatatagagagtctgcagatgttttcttacaggacaaaagggcccatagt
ctgttgaacagcagaaaaagaagagccaatagcttttttgaagaatttaaagcaggatcacttgaacgagaatgtattgaagagatctgttcctatgaag
aggccagggaaatctttcaggatgacagacgcacaaaagaatattggaaagtatacacagatggagatcagtgtctctccaacccgtgcatgaatggtgg
aacctgctttgaccagcaccagtcatatatctgtacctgtcccatgggctatgagggtcggcactgtgaaacaaacttgagggacatgctgaaatgcatt
tatgacaatgggcaatgtgaacatttttgccatgacaactcatcgacaagccgccagtgctcctgtgctgaaggctataaactgggagctgatggtctat
cgtgtgaacctacagttaattatccttgtggcaaaattccagtactgaaaaatgtaaataaacgtgcacggattgtgggaggtgatatgtgtccaaaagg
tgaatgcccctggcaggctcttctgatgtataacgaaatattcatttgtggtggaactttaattgccccaaactgggtaatcacagcagctcactgttta
aagccact
3' EST: CX965623 [+]ttgggtgagctttcctattctttatttatgtcagtgattgactgtactaaaggcattgtgatcatatcatattaatcttgcataggagatgtagctttgc
aaaggacatcagttgaaggcttttgttcttatgcaagagttttgtgtcatacttatttaagcctctggttgctcatccatgttttcctttatccattcag
tgtacctggataccctggtgtacacaccatatttctcttttttagcacatcctaggccccaactgacaataccagtaaggaaatgggtattcttgtactg
tgtagcatgtggccccccactgtcccctttgcaagagtccttgctgccatcagtgtatcctgcacagaacatgttttgtgaaatattcatttgagtctgt
ctgatgcagtcctgtgtcttgactcggggcagctgtactctttgcagtagttcaggagttgccccactctcaagcaggcgtccccagccactgactgtag
agtatctaatcgataagagctcttgtacagcaaactgtttctcaggcaaacataaagggaccacatagtctgtatagttgacaggcgtagtcagtttaag
gagggctatatcattgtcattatttgttttagacccataataatgttcatgcattattattttagaaactttgctctcttgctcagttccctctggtgtg
ccaattctatgttctcctagcactaccgtaagtttgttttctggaagtggctttaaacagtgagctgctgtgattacccagtttggggcaattaaagttc
caccacaaatgaatatttcgttatacatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: