XB-CLONE-3564186
Clone Name: IMAGE:7709866
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Gene:
hmmr
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965752 [+]
ctcactgttggacataaatatagcagaaagtatatgatacaaggttttgagctgtattaaaaagggcagagatttgcgggaggagggggttattcttcta
ctttacagagcgctggtaaggccccatctacaatatgctgtgcagtatttgtgtccagtgctatttgtctccagggatagaagtttccaaaggagggcaa
ctaaactggtttaaggtatgaaaagtcttggttccgaagtaagactggccaagttgggtttgtttacactggagaacgggcgcttaagggggatataact
atgtataaatatataaggggatcatataataatgtctctttaccagtaggtcattccagtagatgtgagggcacccttttcaattagtagaacagatgtt
ttatcttaatattcagtaagggtttttataaatgtgtttgtagaataagggataccatcggtagtagtagcactgtaatactagcacctaatgctagtta
ctacttgtacttagtaatgaagtcacaaataagttatataaatatattatacgccagtaaagctagccccatggaatacagaggcattgcagtgtcatga
tgccatcttggttataataggagaagcaacataatctctagagtgcacgaggagagggacaggagggcggacaacgatacagtggcatcctatgtaacct
gcgcatcatcagaggctggaaaggttgggcattgctagcgtcactctgcaagctggaggggtggatggggaggcaaccagttagcagggacgtaacccca
ataaattatatacagcaatgctaaca
3' EST: CX965751 [+]gcgcacttgactgaaatccagggtgtgaagagattcgatccatccaaagctttcaggcatgatgtcaaagagaatgtgatgccaaaagctcctttacggg
aaggtacgtttccacttaaggggtgttttgttagaaactgtaaatcattagttagaattagaaggaatttctgggccctttaccactttatgggggccac
gttcagggtgctaactgtctaccaacaggctttactgagaggccagcctctatacttggaagtgaatgcatagtataggattggtgatgtgtgggacctt
tgcaaacacaggcatcttcctaaagataggtagcataggtgcgatggttgcattgccatcttcttctttttctagtttcctgtttacacgcaaattgagt
ttgtccccccatgaactattatacttaatatgtttgtttttcacaggaaacaaacgctgaatggacaataactgtgactctgacccacaatcctgcctcc
tgattttatatgttcttttatttttatatatatttggggaggaatactgctgaaaacatccattctaaaccatttgtgctttttactgtcaaatttatca
ttgtagtctattttttcacaactttttatcatgattttatgtgttaaggaaactactctaaacatgcccgcttgcttttagatatatatgacttanacga
agatatcttattatttatgccttgttgtttctgtctggaactttatctttttctggacaggaaaaagtcgatatctagtatgaatattacaggtaaaaat
aaaactagcaagtccttaattacaaatatgtcacacatat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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