XB-CLONE-3564091
Clone Name: IMAGE:7710018
|
Gene:
slc31a1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX965816 [+]
ttattaaaaggttcaataattattattatttttcactattggaaataaaggcaatttataaaaaggtcacatgatgcagataaaaccataaaaaaaaaaa
tcttaaataaaaaaaaatgaaatcggtaacattgcgaataattctgcaattaacatgaacatgtgatcgccttgactccgccccttggcggcacatctaa
atattgggttataattggtcgctaagggatgggattagaacttctgccgtttggggtaccgagggggcggggcttaaagctccagactatcatagggacg
attaaagggcaagttaacctggcgagtgtatgaaacagacacacaatgttacagttgaacatcggctttactgccacacggactcgttatacttccatgg
aaagttccgtagaaatcggatgtggggataacgggaatcgaacatgtgaagtcgtagggctggctggaaggtcacatgacgtacagaccggccaatgaga
gtggtggtgcttaggccaggcttcagatgagacaaagacttgattggctgcagtgacactgtagggcgtgtcttccaggagccaccgtatgattggcgca
gaggaagaggagggaagggggtgaggctacatggtcatgtggttcatccactttatcctcaaaggaaagtgtcactgacccccgctagaggcaaataaag
gcactctgtatgtagggttaccccaatacccccggtaccaacatctaggaatgccgatggaggcggggcacagagctccttccctaacgagcgctcgtta
atggcaatgttccgtgatgtccaccaccaccgccttcttccagctgaacaggaagtagcccgtcccgg
3' EST: CX965815 [+]tctatcaggtgagtctgactgtcgcttcggcccaacatggatcactcccaccacacggtaactaccagcgacccccacgggggccaccaccacccaacca
catccgggaaccacggcgaccacgggggcagcatgcacatgatgcaaatgaccttttactttggatacgagaatgtggaggtgctgttcaccgggttggt
cattaactctgcaggagaaatggcgggtgcgttcgtggcggtgttcctgctggcgctgctgtacgaggggctgaagatctcgcgggaggccttactcagg
aagtcacaagtcagtattcgctataactccatgccggtgccggggcccaatgggaccatactgatggagacccacaaaacagtagggcagcggatgctga
gtgtgccccacctgctgcagaccctactgcacataatccaggtagtggtgagctacttcctgatgctgatcttcatgacgtacaacgcctacctgtgcat
cgcggtggccgccggggccgggacgggctacttcctgttcagctggaagaaggcggtggtggtggacatcacggaacattgccattaacgagcgctcgtt
agggaaggagctctgtgccccgcctccatcggcattcctagatgttggtaccgggggtattggggtaaccctacatacagagtgcctttatttgcctcta
gcgggggtcagtgacactttcctttgaggataaagtggatgaaccacatgaccatgtagcctcacccccttccctcctcttcctctgcgccaatcatacg
gtggctcctggaagacacgccctacagtgtcactgcagccaatcaagtctttgtctcatctgaagcctggcct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: