XB-CLONE-3564059
Clone Name: IMAGE:7710164
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Gene:
XB941699
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965837 [+]
caatttacaagcaatgacagcacagagcaggtaggtgggaagtgcttaaacttagggaaaacgccactgaccccctataagctgaatgacatattagcac
attaattaatggaactctttatatgaactgcttattatagagtaccattagctgtttatattgcaaacaaaaggggtatggccaaaattctggtactgag
tacttctgttcgtgggggccccatataaatatatatatatatatatataaaaacttgtgcggagccccaaagtttctgatggaagccctgggtgccagtg
taaacttgtttgtttattgcaaatgcccatgtgtctcagcatttaggttttgagattctgtttgtgttggctgttatttgtggtaccgacctttgccact
aaacagaatttttaggattggtactggaaatcttttacatctttcaatcattccatttagttagagtaacctaattgtgctctgttcctaattccccctt
ttagctgccttttccttatcagaaaataaggcatgctgctttgatccttcaaggcaaaatttacgccaatattgaaaaaaattgtctgcataaaattcat
tacacagaatggcatgcggtacactgaggtcaccagtactgatgtaatcagccccaggtctggcacaaagatcttattgtaatcatagcttttgggggag
gttaaacttgtataaatcatagcttttgtggaaaataaacttgtagttctgctggaggaggtaatcatttgggatgctttttatgtagttggtaatactg
gtagtactgtaaagaatgtaaaaaagctaaaatggtgccaa
3' EST: CX965836 [+]agcttggaacgtctgtgggacacatttttgattattagactgagacaagctttactatattatctactctccaaaaccacaatctggctgtaaggatgtc
aatagcggctgttttcagaataaatgtctttgctcgtcaaaccccttggtgtgcattaagtgaggctgtaatttctgctaatattgtcacttcaagaaga
aggctgcaccgcaaggccttttatcagtcacaacagaagggacataatgaaatgacaaatgtgataacccagagacatggaactattctgacaattggaa
ttaacaggccagaagccagaaatgctgtgaatccagagacagcttcccagcttgttgaggcctttaatcagtttgagaatgatccaggtctgactgcggc
tgtagtccatggaataggaggtaatttctgtgctgggcttgatttaaaggaaatagctcacgatgcaagtgcattgaatgtagagcaagatgtcactaaa
ggacgtggcccaatgggcccatcaaggatgcagtttaccaagccaataattgctgccgtgagtggttatgcagttgcaggaggaatggagttatccctct
tggcagatatgcgagttgtagagagaagtgcaatatttggaatcttttgcaggcgttttggtgttcctctaattgatggtgggacagtaagacttcccca
gctcattgggctttcccgagccctcgatcttatccttacaggtcggccaatcaattctcaagaagcatatgaatttggattagctaatcgtgtggtacct
gatggccaaggcttacagtgcgctatttgagtagcacacagtatatca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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