XB-CLONE-3564015
Clone Name: IMAGE:7710216
|
Gene:
eef1a1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX965866 [+]
atttagaaattactggatttaaaacactaataacttaaaaactgccacaaacaaatgtgtccacgagcatttttttccttctgaagcttttgcgatgcat
tgtaatcattaaccagtctttgactattaaactctgatggccaattgatagaaagagttctgagaccaatcctccaccactggttatgactgaggtgaga
gatgctggaaatattcatttgcctttctgtgctttctgagcagactttgtgactttgccagctccagcagccttcttatcgaccgccttgatgactccta
cagcaacagtctgcctcatgtcacggacagcaaaacgaccaagaggggggtagtcagagaagctctccacgcacataggctttcctgggatcatgtcaac
aatggcagcatcaccagacttcaggaacttgggattgtcttccagtttcttaccagaacggcgatcaatcttttccttcagctcagcaaacttgcaagca
atgtgagctgtgtggcaatccaacacaggggcatatccagcaccaatctggcctgggtggttcaggatgataacctgtgctgtaaaggtaccagcttcca
ttggtgggtcattcttgctgtcaccagcaacgttgccacgacggacgtccttcacagaaacgtttttcacgttaaagccaacattgtcaccgggcatggc
ctcgctaagggcttcatggtgcatttcaacagatttaacttcagttgttacattaacaggggcaaaagtaaccaccatgcctgggcttaggacaccagtc
tccacacgaccaactggtacagtacccataccgccaattttgtag
3' EST: CX965865 [+]ggtgaatttgaagctggtatctcaaagaacggacaaactcgtgagcatgccctccttgcctacactctgggagtaaagcaactgatcgttggtgttaaca
aaatggattcaactgaacccccatacagccagaaaagatatgaggaaatcgttaaggaagtcagcacatacatcaagaagattggttacaaccctgatac
tgttgcctttgtacctatttctggatggaacggtgacaacatgcttgagcccagcgccaatatgccttggtttaaggggtggaaaatctcacgtaaagag
ggatctggcagcggaactaccctgctggaagctcttgactgcattttgccaccaagtcgcccaactgataagcctctgcgtctgcctctgcaggatgtct
acaaaattggcggtattggtactgtaccagttggtcgtgtggagactggtgtccttaagccaggcatggtggttacttttgcccctgttaatgtaacaac
tgaagttaaatctgttgaaatgcaccatgaagcccttagcgaggccatgcccggtgacaatgttggctttaacgtgaaaaacgtttctgtgaaggacgtc
cgtcgtggcaacgttgctggtgacagcaagaatgacccaccaatggaagctggtacctttacagcacaggttatcatcctgaaccacccaggccagattg
gtgctggatatgccccctgtgtggattgccacacagctcacattgcttgcaagtttgctgagctgaaggaaaagattgatcgccgttctggtaagaaact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: