XB-CLONE-3564006
Clone Name: IMAGE:7710026
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Gene:
MGC69416
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965872 [+]
caaaagtgaacaaagttctgcttgtgggtgaggtgtggggatatttaggtcaaagggctgcactcctgccgatcctgtctgagctgtcaattccctgccg
gggatagaaaataaacagaagcctcccagccctacccctgtctgcagcaactttgaaggggaaatatcactcaggtcaggctggagccatgttggccgct
gatccaatgacaatactcctctcagcctttatttgccttcttctgggatttgttttgtttggcaggaaaaggaatgtttgtcagaatttccctccgggtc
caagagcccttccagttattggcaatttactccttatggataggaaacaaccctataaaacactgatggaggtatctaagaagtatggccctatattcag
cgttcgggcagggccacagaagatggttgtcctgtgcggctacgatacagtgaaagatgcccttctcaattaccccgatgagtttgctgaccggcctgcc
ctgcctttgtttgacgaagtagtcaaaggccacggtattttcttttccaatggcgagaactggaaggtgatgagacgctttggtctctcggcgctaagag
actttggaatggggaagaagacgatagagagcaagatcaatgaagagtgtgatcatttagtgcaaaagtttaactcctacggaggaaaaccctttgacac
cactatgataatgaacgcagccgcagccaacattatcgcttccattttactcagtcatcgatttcaatatgaaaatccaacgttgctgagactcctgaaa
ctggtgaacgaaaacatacggcttatggcaagcccgatagccctgctctacaacactttcccct
3' EST: CX965871 [+]gactttccaatcagttttatattcgtgcataaaacatttagcagcttatatcttgttacagaattggaattacacaaaaattctaccatattttgaaagc
ttaggttgttctgaaaaaaacgatatattgttttcctgggtaaactaaaagttcccccgaggaaaggcccctaaagtgaaacagtctggcaatacaagtt
ccactttgaccaaaatggctggcagtaaaagggttaaaatcaacacctaattggttgctatgagttaacagaagtgggtaaaattaggacttttattaca
ttaacccaaactgataaataaaactcaaaataacaaagtacatttcaagaggaatattcagttggtcaacgcactagcctcaggaacgagctaaagcaca
aacagtatggggcctagggggcacagttccaccgacatcaggggtgagatcaagttcctccccaggaggcgcctggaaggtgaagttctgcaggaggctt
gtgaagaacaggaacagttccattctagccaacgtctctccggcacagcttctgcgacctgctgagaagggaaggaacgcctcgtttttcacaaaatgtc
cttctgagtcgaggaaatgttccggataaaattcgtttggcttctcaaagtaattctcatctttcagtacagatgccagcaatgggattacataggtgcc
tttgggtaggaaaaatcccctgaaagtgacatccttggtagttgcatgggggccattcattggagtgatgttgccaaatctctggatctcatgaagtaca
gcatcagtatagggcatctctttcctgtgctctgctct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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