XB-CLONE-3563831
Clone Name: IMAGE:7710281
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Gene:
hacl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX965988 [+]
aaagtatatgtttggcattgtcggaatccctgtcattgaaatagctgtggctgcacaggctgctggtattaagtatgtggggatgagaaatgaacaagcg
gcttgctatgcagcctctgctgtgggctatctaactgggaggccaggagtatgtcttgttgtctctggaccaggcctcatcaatgcattaagtggaatgg
caaattctaacatgaactgctggccactcattgtgttggcaggatcatccgataccaatcaggagacaatgggagcttttcaggaattcccacaggttga
aacaagccggctatacagcaagttttctgcaagacccagtagtttggaggctattccagttgtcattgaaaaggctgtaagaagcagcatctatggccga
cctggttcctgctacgtggatattccaggagactttgtaaacaaaatggttaacaggagtgatgtgaggtacactgaatgctgtttgccaccccccacca
gcctagcagatcccaccgctgtacacaaagcagtgctggagctaaagaatgcgcaacagcctcttgtcatagtaggaaaaggtgctgccttttcacgtgc
agaaagaagcataagtaaattggtgcaagattgtgggctaccattcttacctactccaatgggcaagggagttgtgccagataaccacccaaactgcgtg
gcagcagccagatccagtgcactacagaatgctgatgttatactgctgctgggagccagactcaactgggattttgcactttggac
3' EST: CX965987 [+]ctttttcagttcaagttaactgccctagtgaaggaataattatcttataagaaaacaaataaatgagcactaaatttagtgaaacattatgtctttattt
tgcacccaaacgctgtattaggctcgaaatttattaggtgaaatcatttaaaaaaatatttttggtttgttaatttatactactatttacacagaagtgt
tctattttctgtttggaagcattcttttctattataaaaatactttttagtattttcatttatcggtaattaatagaatgtactaccttcaaataaaaca
aaaaggcatgaatactaagtgcaatttcataaaaggaaagcaatcacaggttggaccgggtcagccagtggaactcctgttttttcctgtctgaaactgg
atctatcataacattgatcaatgaaggaacagccttttctgcaaagctggatttcaaagcattctgcaactcttcaggggtacgagcaaagtatcctttg
ccaccaaaggccttcataacctgttcatagtgagcatttggcattagtgccacaggaggggccatggttgcagggtccccagactccatcatttcattcc
atgtgccctcatcaaatccattgtaaatgccattgttattaacaacaatgattataattggtagtttatacctgcacattgtttcagcttccatgccaga
gaaaccaaaagcactgtctccctcaatacaaaccacacgctgttcaggacattcatctttggttatcatcgctgctgctatggcaaatcctagtccaact
cccatggttccaaatgtgccagcatctaacctgtgtcgtggatggtagttcggcagtacagtgcgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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