XB-CLONE-3563760
Clone Name: IMAGE:7710527
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Gene:
gnl3l
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966040 [+]
aggcaacgtacaagggccagggtaaaggatctgtgcagcatgggaagaatgtcaagaaagaccctggagtgcccaacttgtcccacttcaaagagcaggc
aaagagagaggctgagcagaagaagaaaagggtggaagagatgagagaaaagcagaaagctgccagacagaaagaaatggctaagaggaggagcctgggt
acatttcagaacgatgttttgaagcgccaaagggagtttgagcaaaaggaggctgatcttaggagtctggagaagcatgagcagctggagaatgagaatt
ccaggaaggcttactacagggagttcaaaaaggtggtagaagcggcagatgttattttagaagtgctggatgccagggatcctttgggctgccgatgtcc
ccaagtggagcaggctgtagtacagtctggcacagagaagaaactggtgttggttcttaataaaatagatctcgtccctaaacctgttgtggagaagtgg
ttgaagtacctcaggaatgagttccctacagtggccttcaaagcctccactcagcagcagaacaagaatttgcaacagagcagagtgccagtcaagcagg
catctgaggatctgctgagctctggagcctgcattggcgctgattctctaatgaaactcctgggcaattactgtcggaacaaggacatcaagacatctat
aagtgtgggggttgtagggtttccaaacgtggggaagagcagtttgattaacagtctaaagagagcaagggcctgtaatgtgggtgccacacccggcgtg
accaaatgcctgc
3' EST: CX966039 [+]ttaaacccagagaagcaactcaataaacagtttaggtcgaagaaaacagtgagggttgctgctcctgttttgttgcataagtggctactcagcattatta
tagtgacccagcacagtccagcatttaggataatgacaagggtcctgtgtaccggcggggctgcaaggatgatgcacccttatggtcaacgatgggcttt
ataaaagtccattttccctctcagtcatcgctcagtccaaaattcattgctagcgtaagcgaatctgacagttttgtggcaattttgttcgctcgcttct
gcaactttttcctcttcttgctggcggcgagcagggcctgtccttgctgcaatgggtgaatagactgaatctcctgcaagttcggggctttgggaactac
ttgttctggttcaagctggactttgctttttagggctttgatttccacagtcatgggaccaaactctgtatctatgtcctgttccatggaatctgtgggt
ttctcatcctcatcacttaactcatccagttcaacatgctccacctcttctccgacaccagctgtgagcccagagacctccataacaatgccctctggga
tagactggacctttaataaagactcatggttgtccatctccagggcctcaaaatcaaatgccttccccatttctgtgacaatctccgcactgacgtgtgt
aggcatggtgtgcgtctctggnggatgggtgaagtaactgatcttaccactcacccaatctgtaagaacagacttggcagccttctcatgatccggtgtg
ccacctttcttcagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: