XB-CLONE-3563581
Clone Name: IMAGE:7710401
|
Gene:
XB5899916
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX966160 [+]
cccttctccatcatctgcatcatcgccaacctcttcctcctcttcccaggactcagtattgatccaattcaagatgcaagccaacagatgacccctgagg
ttctttacctggggggagtcattggagggggcttcttggttctgattcctgccattcacattctgtccacgggacaaaaggaaaactgctgcaataatcg
ctgtgggatgttcctgtccatcatctttgctgttattgggttggtgggagccgtatacgccctggcagtgtcggccctaggaatggtgtatgggccagtc
tgtgagtacccggatcccaaaagtggaaatctgacttggggccagcccttcaaagataaccttgaaaaattcagtaatgagagttacctgttcaataagg
accagtggtatttctgtgagaagccagaccacgtggccccatacaacattactctcttctccatcatcctggctgccagcggggtagaagtcattctgtg
tgttattcaggtcataaacggcctggccggctgtatctgtgggacctgccagaaaaagaactgagattgccatgtcccactcccccacaatcccctctgt
ccgcagttgtctcctccagaggctggttcctttgagagtctcccctgccctcatggtctgacccgccagcgggcttggcattgccaggggtaggagcatt
actccctactgtaaatcccggatgtagatgtacgtaggcagaatgaaagggtgcccccctcagacgaagccgctcaatacgtctcccatgtgagggatgc
tgggagctttgcaccgggg
3' EST: CX966159 [+]ccggtgcaaagctcccagcatccctcacatgggagacgtattgagcggcttcgtctgaggggggcaccctttcattctgcctacgtacatctacatccgg
gatttacagtagggagtaatgctcctacccctggcaatgccaagcccgctggcgggtcagaccatgagggcaggggagactctcaaaggaaccagcctct
ggaggagacaactgcggacagaggggattgtgggggagtgggacatggcaatctcagttctttttctggcaggtcccacagatacagccggccaggccgt
ttatgacctgaataacacacagaatgacttctaccccgctggcagccaggatgatggagaagagagtaatgttgtatggggccacgtggtctggcttctc
acagaaataccactggtccttattgaacaggtaactctcattactgaatttttcaaggttatctttgaagggctggccccaagtcagatttccacttttg
ggatccgggtactcacagactggcccatacaccattcctagggccgacactgccagggcgtatacggctcccaccaacccaataacagcaaagatgatgg
acaggaacatcccacagcgattattgcagcagttttccttttgtcccgtggacagaatgtgaatggcaggaatcagaaccaagaagccccctccaatgac
tccccccaggtaaagaacctcaggggtcatctgttggcttgcatcttgaattggatcaatactgagtcctgngaagaggaggaagaggttggcgatgatg
cagatgatgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: