XB-CLONE-3563563
Clone Name: IMAGE:7710451
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Gene:
fsd1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966172 [+]
gctctgcaaatgtctgaattctgacagtttttctattttttgatctaaccttaacttgtgaacacgctaatgcttttatcagctacatgtatggcccagc
cttgccacatcagagttgcagataagtaggctaaaaatactaaaattgggtaaatagagagtgttggtgccctgagattaatttaaggtcacaataaatg
gggtaagctccgcaactgcatatattgtgtgtgcgcggctccgttacaactatgtctactgaaggtagggacactgccagtcccatggaaggcttgcacc
accagtgcagtgaatgcattatatttacagatgtgcagtgaagatagttcggctctgagactaattccacacatggctgatgcagaaaggagctgatgac
aacaaataaaaagcaggtggagaaacagcctaagatggagaaagttcttggggatgagcttatagatgtttagaagctacacatagagcttatgcataca
aacaagctgacagtatcgcgatactacatgtaacttatatttatttaaggcataatcagcagaatgccacacttagcgtactgtccatccatctcttaca
ggtgcagaggatatcatggaatatttgttgtagaaccctaaattatgttcaagttttttttaataggtcccctaaaatactgggtggactttaagcttta
atgcattgtaaagggagagaaaaggagagatctaggtggggaaatgaaggaggtgggtagatcagatgaaatcaagtgaggctggtgcttgagctgctgg
tggcacta
3' EST: CX966171 [+]ttgaagttatatgacaaacacattgaccagaaatgtaaccataaaatatcagatctaaatcttaacattaaactcatcctgaacacaaagactaggcaag
ctgtaccttaatagtatgatggctttagtacaatatttgtattgttgctaattaccttctgtcaaccaacaggtatggtgtggaagtttctcagtgtata
ctggactccaggttcctagctgtgtgaaaagcctccagaaaaggaatagtgccaccagcagctcaagcaccagcctcacttgatttcatctgatctaccc
acctccttcatttccccacctagatctctccttttctctccctttacaatgcattaaagcttaaagtccacccagtattttaggggacctattaaaaaaa
acttgaacataatttagggttctacaacaaatattccatgatatcctctgcacctgtaagagatggatggacagtacgctaagtgtggcattctgctgat
tatgccttaaataaatataagttacatgtagtatcgcgatactgtcagcttgtttgtatgcataagctctatgtgtagcttctaaacatctataagctca
tccccaagaactttctccatcttaggctgtttctccacctgctttttatttgttgtcatcagctcctttctgcatcagccatgtgtggaattagtctcag
agccgaactatcttcactgcacatctgtaaatataatgcattcactgcactggtggtgcaagccttccatgggactggcagtgtccctaccttcagtaga
catagttgtaacgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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