XB-CLONE-3563542
Clone Name: IMAGE:7710501
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Gene:
smarce1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966186 [+]
attggcaagatcattggtgggatgtggcgagacctaaccgatgaggagaagcaagagtacttgaacgaatatgaagccgaaaagattgagtacaacgaat
caatgaagacttaccacaactctcctgcttatcttgcctacatcaatgccaaaagccgggctgaagctgccctcgaggaggaaagccgccagaggcagtc
gcgcatggacaagggggagccgtacatgagcatccagccggccgaggatccggacgattatgacgatggtttctccatgaagcacagcgccgctgcccgt
ttccagaggaaccaccggctcatcagtgaaattctcagcgagagcgtggtgcccgacgtccgctccgtggtcacgaccgctcgaatgcaggttctgaaaa
gacaagttcagtctctcatggtgcaccagcggaagttggaggcagaattgttacagatagaggatcgccaccaggataagaagaggaaattcttagaaag
cacggagtcctttaacaatgagctgaagcggctgtgtactttaaaggtggaggtggatatggataagattgcggcagagatagcccaggcagaagagcag
gcaaggaagcgccaggaggagcgggaaaaggaggccgcagaacaagcagagcgcagtcagaacgcagcagccgtggcggcagcagcagcggcggcggcag
cagctgtggcagctgtggcggctgctgaggaagagcaagagcaagaggtggcaaaaccagaagaaaagagtgaagagga
3' EST: CX966185 [+]tatagggcgagaggttatttggaattcatttgctacaaaatgtaaaagtattaaaaaaaaaagttaattttctatttctccgacctatccagctcccaag
ttgatctgcagcgctggcaactgcttgactgggtaataccatatgccccaccccccatggggctgaaggaaacctctctctgggactcctaattttgccg
tggggagtgaataaaagccgaggggcccaggttaagcccgtgtgtgtccccccccaacgctagccgaatacatttaggaaagcgtttccccactgccccc
catgcagacacagattacaacacaacaaaacagatgtaggtgaatggggagtgttcagttcttgcggtcggtctcttccgctacagagtctgtggtgggt
tcctccacggtgccgctgttgctctccgacccagtgttgctgtcgcttgtaccttcatctagggtgctgtccaccgcctcctgcccgctctccttgtcgt
cgggggtggaggtcccctcttcgccattttgttggctctcgctggtttcctctgctgggggcagggatggggtgtcttctgtctccataggggcctcctc
ctcttcactcttttcttctggttttgccacctcttgctcttgctcttcctcagcagccgccacagctgccacagctgctgccgccgccgctgctgctgcc
gccacggctgctgcgttctgactgcgctctgcttgttctgcggcctccttttcccgctcctcctggcgcttccttgcctgctcttctgcctgggctatct
ctgcccgcatcttatccatat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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