XB-CLONE-3563483
Clone Name: IMAGE:7710460
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Gene:
nr2f2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157199 [+]
ATTTATTCCCCCCCTTTCCCCCCCTCGACCCCTCTCTTTTTTTTTCTCCCCCCTAAATCCATTCTGTCTCGCCTGATCCCATAAGCGTGAGAAGGCAAAA
GAAAGGAGGGAGAAACGGTTCCCAATAGATATGGCAATGGTAGTGGGTGCGTGGCGAGACCCTCAGGACGATATGCCAGGAACTCAACCTTCACAAGCAC
CTCCAGGGCAAGGGCCAAACGGGGCCCCGCACACCCCGCAGACCCCGGGCCAAGGGGTCCCCTCTACTACCCCAGCCCAGTCCAACCCATCCAGCCAACC
GAGCCAGAACCAAGGGGAGAAGCAGCAGCAGCAGCAGCACATCGAGTGCGTGGTGTGCGGGGACAAGTCCAGCGGGAAACATTACGGACAGTTCACATGC
GAGGGCTGCAAGAGCTTCTTCAAGCGAAGTGTAAGGCGAAATCTCACTTACACGTGTCGTGCCAACAGGAACTGTCCCATAGACCAACACCACCGCAATC
AGTGTCAGTACTGTCGCCTCAAAAAATGCCTCAAAGTTGGCATGAGACGGGAAGCCGTACAGAGGGGCAGAATGCCACCCACACAGCCTACACACGGGCA
GTTCGCCTTGACAAATGGAGACCCTCTCAACTGCCATTCCTACCTATCCGGATATATATCGTTACTCCTGAGGGCAGAACCCTACCCGACCTCTAGATTT
GGCAGCCAGTGCATGCAACCCAACAATATCATGGGCATCGAGAACATTTGTGAGCTGGCAGCCAGGATGCTGTTCAGTGCTGTGGAGTGGGCAAGGAATA
TCCCCTTCTTCCCAGACCTGCAGATCACAGACCAGGTGGCATTGCTCAGGCTGACCTGGAGTGAGTTGTTTGTCCTCAACGCTGCCCAGTGTTCCATGCC
CCTCCATGTGGCCCCTTTACTGGCCGCTGCTGGCCTGCACGCTTCCCCCATGTCTGCGGACAGAGTGGTGGCATTTATGGATCACATACGAATCTTCCAA
GAACAGGTCGAAAAACTGAAGGCTTTGCACGTTGACTCTGCAGAATACAGCTGCCTCAAGGCCATAGTACTCTTCACTTCAGATGCCTGTGGTCTGTCTG
ATGTGGCACATGTGGAAAGTTTGCAAGAAAAGTCCCAATGTGCTTTGGAAGAATATGTTAGAAGCCAGTACCCAAACCAACCAACAAGATTTGGGAAATT
GTTACTTCGACTGCCCTCCCTCCGCACTGTCTCCTCCTCTGTCATAGAGCAGTTGTTTTTCGTCCGGTTGGTAGGTAAAACCCCTATAGAGACCCTAATT
AGGGATATGTTACTCTCTGGAAGCAGTTTCAATTGGCCCTATATGTCCATACAATAAAACATTAAGTATTGAAAGGGAAATAAAGGAGGGCAAAAAAAAA
5' EST: CX966229 [+]atttattccccccctttccccccctcgacccctctctttttttttctcccccctaaatccattctgtctcgcctgatcccataagcgtgagaaggcaaaa
gaaaggagggagaaacggttcccaatagatatggcaatggtagtgggtgcgtggcgagaccctcaggacgatatgccaggaactcaaccttcacaagcac
ctccagggcaagggccaaacggggccccgcacaccccgcagaccccgggccaaggggtcccctctactaccccagcccagtccaacccatccagccaacc
gagccagaaccaaggggagaagcagcagcagcagcagcacatcgagtgcgtggtgtgcggggacaagtccagcgggaaacattacggacagttcacatgc
gagggctgcaagagcttcttcaagcgaagtgtaaggcgaaatctcacttacacgtgtcgtgccaacaggaactgtcccatagaccaacaccaccgcaatc
agtgtcagtactgtcgcctcaaaaaatgcctcaaagttggcatgagacgggaagccgtacagaggggcagaatgccacccacacagcctacacacgggca
gttcgccttgacaaatggagaccctctcaactgccattcctacctatccggatatatatcgttactcctgagggcagaaccctacccgacctctagattt
ggcagccagtgcatgcaacccaacaatatcatgggcatcgagaacatttgtgagctggcagccaggatgctgttcagtgctgtggagtgggcaaggaata
t
3' EST: CX966228 [+]tttttttttgccctcctttatttccctttcatacttaatgttttattgtatggacatatagggccaattgaaactgcttccagagagtaacatatcccta
attagggtctctataggggttttacctaccaaccggacgaaaaacaactgctctatgacagaggaggagacagtgcggagggagggcagtcgaagtaaca
atttcccaaatcttgttggttggtttgggtactggcttctaacatattcttccaaagcacattgggacttttcttgcaaactttccacatgtgccacatc
agacagaccacaggcatctgaagtgaagagtactatggccttgaggcagctgtattctgcagagtcaacgtgcaaagccttcagtttttcgacctgttct
tggaagattcgtatgtgatccataaatgccaccactctgtccgcagacatgggggaagcgtgcaggccagcagcggccagtaaaggggccacatggaggg
gcatggaacactgggcagcgttgaggacaaacaactcactccaggtcagcctgagcaatgccacctggtctgtgatctgcaggtctgggaagaaggggat
attccttgcccactccacagcactgaacagcatcctggctgccagctcacaaatgttctcgatgcccatgatattgttgggttgcatgcactggctgcca
aatctagaggtcgggtagggttctgccctcaggagtaacgatatatatccggataggtaggaatggcagttgagagggtctccatttgtncaggcgaact
gcccgtgtgtaggctgtgtgggtggcattctgcccctctgtacggcttcccgtctcatgccaactttgaggcattttttgaggcgacagtactgacactg
atttgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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