XB-CLONE-3563442
Clone Name: IMAGE:7710368
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Gene:
adsl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966256 [+]
cagcgtgtccccgctgactcccgggcccgaagaggttatgaggtataggtcgccgctggtctccaggtacgccagccgggaaatggccttcaatttcagt
gacagcaagaaatttcagacgtggagacggctttggttatggctggggcaggcccaacggagtttggggctacccattacagaagaacagatccaggaga
tggaggccaacctggaaaatattgactttaagatggcagcggaggaagaaaagcgcttgcggcatgatgttatggcacatgtacacacgtttgcacactg
ctgccccaaagctgccccagtcattcatctcggagccacttcttgctacgtgggagacaacactgacctgattgttctacgtgatggttttgacctgctg
cttccaaagcttgctcgagttataagccgcttggctgattttgcagaaagatacgcagagatgccaacgttaggattcacccactaccagccagctcagc
tcacaactgtggggaagcgtgcatgcctttggcttcaggacttgtgcatggacttgcgcaacttggagcgagccaggaatgaccttcgcttccgaggagt
gaaaggtactacaggcacacaggccagcttcctgcagctttttgatggtgatcatgacaaggtggaggaattggacagaatggttacttctaaggcagga
tttaaaaggtactgggcttttcagaaaaattccatttcgatgtggctttgcaaaaacaggcaaaactggtgcggtattggtttttatagagttacaacag
aaaccttggtttgccatctctagccatgcctcagtactcttagttac
3' EST: CX966255 [+]gcaaataaatatttctgattgacacatttcacaggtccagctcaatcttcacatccattttactctgataaggactcagcagtggaatcacctcctcctt
taaaaacttcagcacctgctgaggtgcccggcctgtgaaggacttcggatcaagcagctgttccagatgagcatgaattggggcaaagtaagaatcagat
tggattctgaagataaggtcattgtctcctccttcttgttttaccactgcagccgcttgctgggagagaactcggattcgctcatggcagtcctgcctgt
ttcctccattcttcaccattgccataatgatattttctgtggccataaagggcagctcttggcggattcttctttcaatcacttttggatacaccaccaa
tccttccgaaatgttctgcagagtgctaagaatgatatctgcagtgagaaaggcttctgccaaacatactcgtctgttggcactgtcatctagtgtgcga
tcaaaccactgaactgaggcagtctgtagcgggttcatgatgagagtcataaggtggcgagccaagctgcagcagcgttctgagcgcatggggtttctct
tgtagggcatggcactagaaccgatctgttctttctcaaatggctcttcaagctctttcaggtttgccagtaaacggatatcagtgcaaatcttgtgaac
agtcgcccctaaactagccaggacagacagcacctctacatctaccttgcggctgtaagtttgtccagtcactatataagccctaaga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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