XB-CLONE-3563421
Clone Name: IMAGE:7710370
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Gene:
nsmce3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966272 [+]
cttctccttgtcctacatcaagatcttcaacgttgggagccgatacctggtgaacagggttcaggatcacattcagagccgtgcagtgtactacctaatg
aatatccacgtcactccccgctccatttacctttgccggcatggagagagtgagcttaaccttttggggagaatcggtggtgattcggggctgtccacac
gtggcaaacagtttgcacacgccctgggcaactttgtgaagtcgcagcaaattaccgaccttaaggtgtggacaagtcacatgaaacgcaccatccagac
ggcagaagctttaggtgttccctatgagcagtggaaggctctgaatgagatcgatgctggggtgtgtgaggaaatgacatatgaggaaatacaagagagg
ttcccagaggagtttgccctaagagaccaggacaagtatcggtacagataccccaaaggagagtcctatgaggaccttgtacagaggctggaacctgtca
taatggagctagaacggcaggagaacgtgctggtcgtctgtcatcaagcggtcatgcgctgcctgctggcctacttcctggacaagagtgcagagcaact
tccgtacctgaaatgtcccctccacacagtgctgaaactaacccctgtggcatatggttgtaaagtagaatccatctatctgaatatagaggcagtgaac
actcacagggaaaagccatttaatgttgatgtttcccgtgacccagaggaagctttggacactgtgcccgaacatttttaactgtaaagttttttgtata
tttttaattttgttttagagttgc
3' EST: CX966271 [+]tcagtggttacacatctccaggagatttggactttgttctgcaacactgatgtcttaaagtgacagttaaacttgaacgttctgttcacaataactgctg
gttcagctaaaatgccatatgcttgctgttgcttttccaagcactgcagggagtctcagctcagactacttattgtcaggaagagaagttcattttcatg
ttttaataaaatactatttctttgcaaaaagccctttgtgctgctttccttttaatttccactgagcccattcattcatattctagcagcacaactcctg
ctccagtgatggataaattgcaccctgcatcctcctaaaaaggaggtgactaaatgtaccacagggacactaacatctgactgtcctccagataccagag
tttataaaattatattaaagtcactaaacatgattaaaaataaacagacccaattactaaatatacaaacaaatcactagtgggcctgagaactaccttt
tagctaccttccaataaagcataactggggctctgcctatatgggggaatagtagccacaggcaaaaaaaaaaaaaaaaattaaccccactagccattcc
ctccattgtacatccttactgcattcccagtttggcgcctcattagccactgacacttccctcatgttaaaaatcaatctgattggagcaactctaaaac
aaaattaaaaatatacaaaaaactttacagttaaaaatgttcgggcacagtgtccaaagcttcctctgggtcacgggaaacatcaacattaaatggcttt
tccctgtgagtgttcactgcctctatattcagatagatggattctactttacaaccatatgccaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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