XB-CLONE-3563251
Clone Name: IMAGE:7710530
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Gene:
tmem123
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966385
[+]
aaagattgtttgtttgtaagcccactgggtactgattgggtcagtaccatgacgggaggttactaggcatacctatgagccccttgtagcatggtgtatt
catagcacactgagctcaggctgtcacgcggaccaggtctgtataataatgatatggaaaatgcttttcttctgcttcacaagagaaaaatcaatatttg
cacttttacccgtcgcacgataaggaaatgaatcttgccttttgctttgcatccggttctgtacggctgccttttgccttatattttgtctgcgttttat
aattatcagtgcgtttgtctgtgcggtgcagccataggaggcgttagtaacgtatgcaagggtcgggcatgggcattgggcatcccttttcccagggagc
agctgggggcatagattggcattttgattagcagccatgcctccagtgggcaacggtcatcaaaaacgttgttttgcttatggagcctatgctggcatgt
aattgtgtttattggcctgctattaaatacgctccccacatatggcacatatggctagtaaaaaaatctgcacggcattttcatgatttatgttatttgg
ctgaagatcatattcctttttgtgtagcaactaaactgcattatccttacatttgtagcttgtcattctacactgctcctctaggtgtcgctgttagctt
tttctttttttttttattc
3' EST: CX966384
[+]gaaaagtggcttgtctatgtctctataccatggacaaatcctccacgtcttgccactactaacaaatctagtagtttctgtatggttaaggaggtctcag
taaggtacggtctggggaatgtgataacattaacatgatttgacaattcaaatgggcactgataaaatcgttgttcattcatttatcgctaattgtgttt
tgatagaaatatggctgccatttttattttttttttaactttttctctgctgttaattgcggcttctgtcagacttacaggttctgtataacgtgcacat
accttattccatgcctaataaaatgagcagttacgaaaattatccttttaattaaacagaaattcaggatcattgtattcacacattccttaaagccaaa
gacacatataaaaactgcttaaggccatatatatctgagcagttaatcctgtgtttcccattcctttgtgatacaaattcaatccatcccatactgtaca
atggaggaaaaaaaacacaataaaaaccattttacacactgatttaaaaaatacaaactattttccactccaaaaacaataactctgtatcaatctcctc
aattgaaaagggaaaaagaatctttttacccgggtttatacaaaatgtacagaaatccgctgacagatcaatccccgcctcggatcggaagaatgatttg
gccttagctgggggcctatggagacatctctgtggcggtcatgtgataaaggctgcccaacagcaccggtcccatacgccactgactttaatggtatcta
cccattactgcgcacattgngc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: