XB-CLONE-3563248
Clone Name: IMAGE:7710578
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Gene:
ids
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC154891 [+]
CTGAGTGCTCTTACAAGTATGCTTGTTGTGGTGTTCTACAGTACGCAATGAATTTGTTTGGATACCTTCGTTTCCTGATGTGCGCCACCACTGTGTTTGC
TGTCTGGCAACAGCCCTTTTTACCGAAACATACCGCTACAGGTGGAAAGAATGTTTTAATTATAATTGCTGATGATCTACGTACAAGTCTTGGCTGTTAC
GGTGACAGTGCTGTGAAATCACCAAACATCGATCACCTAGCATCACAAAGTATAATATTTACAAATGCATATGCACAGCAAGCAGTGTGTGCCCCAAGCA
GGGTGTCCTTCTTAACAGGAAGGCGTCCAGACACAACCAGGCTTTTTGACTTTAATTCCTACTGGAGAACACATGCTGGAAACTACACAACTCTTCCTCA
GTACTTTAAAGAACATGGTTATGTGACAATGTCAGTTGGGAAAATCTTCCACCCAGGTATTTCATCCAACCATAGTGATGACTATCCTTATAGCTGGTCA
GTATATCCATACCACCCTTCTGCAGAAAAATACGAAAATTCACAGACTTGTAAGGGTAAAGATGGCAAGCTTCATGCCAACTTGGTGTGTCCAGTTGATG
TTTCTGAAGTCCCTGAAGGAACATTACCAGATATACAGAGCACAGAAGAAGCTATCCGACTTTTAAAGACTGTAAAACAACAGAATGCCTCATTTTTTTT
AGCTGTTGGATATCATAAACCACATATTCCATTTAGGTTTCCAAAAGAGTTCCTGAAGTTATATCCTATTGAAAACATTTCATTGGCACCAGACCCAGAC
ATACCCAAGAAACTTCCTCTGGTGGCCTACAACCCATGGACAGATATTCGTAAAAGAGAGGACGTGCAAGCATTAAACATCAGTTTTCCTTATGGTCCTA
TTCCAGAGCATTTCCAGCTTCTGATTCGTCAGAGTTACTATGCCTCTGTCTCTTACCTTGATGATCAGATTGGGCAGTTGCTGAATGCAGTAGAAGACCT
GGGACTGTCTAATGATACCATTATTGTATTTTCTTCAGATCACGGATGGTCATTAGGAGAGCATGGTGAATGGGCAAAGTACAGCAATTTTGATGTGACC
ACTCGTGTCCCCTTGATATTCTATGTCCCTGGAATGACCAATATTCCGCAGCAACCTATTTTTCAATACATAGATCCTTTTAGTACTAACCTACAAAGAA
AATTCCCAGGCAAGTCAAGGGAATATCCTGTTGAGCTAGTTTCCCTGTTTTCCACCATTGCTGACCTTGCCAAACTTCCTGCACCCCCTGCTTGTCCACA
GCCTTCATTCCATATGGAACTTTGTACTGAAGGCCGAAGCCTTGTGCATCAGTTGCACGCATCTGAGAACACGCACGATGATGCAGTATTAGCTGTGGCT
TACAGCAGTTATCCGCGGCCTTCTGATTTTCCACAGTGGAATTCTGATCTTCCTGATTTAAAAGATATAAAAATCATGGGATATTCCATGCGCACAATGG
ACTACAGATATACTGTGTGGGTTGGATATAATTCTACAACTTTTCAAGCAAATTTTAAAGAGATTCATGGACGTGAGCTTTATTTTGTCCTGAGTGATCC
AAACCAAGATAACAACCTATACAATCAATTGTTGCATCTTGATATATATAAGCATTTTGAATTTATGAATAACTGAAATATGGACTTATATATGCATATA
TATTTCTGTACAGTATTAATAATGAATATTTTTGGAAAATTTATTGTTTTTATTAAA
5' EST: CX966387 [+]ctgagtgctcttacagtatgcttgttgtggtgttctacagtacgcaatgaatttgtttggataccttcgtttcctgatgtgcgccaccactgtgtttgct
gtctggcaacagccctttttaccgaaacataccgctacaggtggaaagaatgttttaattataattgctgatgatctacgtacaagtcttggctgttacg
gtgacagtgctgtgaaatcaccaaacatcgatcacctagcatcacaaagtataatatttacaaatgcatatgcacagcaagcagtgtgtgccccaagcag
ggtgtccttcttaacaggaaggcgtccagacacaaccaggctttttgactttaattcctactggagaacacatgctggaaactacacaactcttcctcag
tactttaaagaacatggttatgtgacaatgtcagttgggaaaatcttccacccaggtatttcatccaaccatagtgatgactatccttatagctggtcag
tatatccataccacccttctgcagaaaaatacgaaaattcacagacttgtaagggtaaagatggcaagcttcatgccaacttggtgtgtccagttgatgt
ttctgaagtccctgaaggaacattaccagatatacagagcacagaagaagctatccgacttttaaagactgtaaaacaacagaatgcctcatttttttta
gctgttggatatcataaaccacatattccatttaggttttccaaaagagttccctgaagttatatcctattgaaaacatttcattggcaccagacccaga
cata
3' EST: CX966386 [+]tttaaaaaaacaataaattttccaaaaatattcattattaatactgtacagaaatatatatgcatatataagtccatatttcagttattcataaattcaa
aatgcttatatatatcaagatgcaacaattgattgtataggttgttatcttggtttggatcactcaggacaaaataaagctcacgtccatgaatctcttt
aaaatttgcttgaaaagttgtagaattatatccaacccacacagtatatctgtagtccattgtgcgcatggaatatcccatgatttttatatcttttaaa
tcaggaagatcagaattccactgtggaaaatcagaaggccgcggataactgctgtaagccacagctaatactgcatcatcgtgcgtgttctcagatgcgt
gcaactgatgcacaaggcttcggccttcagtacaaagttccatatggaatgaaggctgtggacaagcagggggtgcaggaagtttggcaaggtcagcaat
ggtggaaaacagggaaactagctcaacaggatattcccttgacttgcctgggaattttctttgtaggttagtactaaaaggatctatgtattgaaaaata
ggttgctgcggaatattggtcattccagggacatagaatatcaaggggacacgagtggtcacatcaaaattgctgtactttgcccattcaccatgctctc
ctaatgaccatccgtgatctgaagaanatacaataatggtatcattagacagtcccaggtcttctactgcattcagcaactgcccaatctgatcatcaag
gtaagagacagaggcatagtaactctgacgaatc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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