XB-CLONE-3563148
Clone Name: IMAGE:7710444
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Gene:
trim69.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966453 [+]
ctccagtctgcttctattctatgtatacttcttcagtctgcctggcacctttagattcttagggacccagtaatctttgggaagtactcttgatttactt
tactactgtgatttctcaagtttcttccttgagacttctgcaagtgagaaattacaagcaagaggaccaatgagcgcaaccttggactcaactgtaagtg
agcctgctgtgcttactgaatgggcttgttacaaataaaagtgctgctatgcaatgtagtcttcaaaagatactatagacttaaaaattaagttactatg
gggtaaagattaaatagtagagtactaaattaaagtaatatcataaaatatgattgttatacttttataaaatattttgttagtctgtattagctgcttt
gcagccagttaagaccttaatatttatgtagcctaaatatctatctatgaatagtccaagttaattccaagtaagatcatatgcattcatttttagatgt
cataacagaatgaaatcattattaacatacagaggaatcattttaataagagagaatatattttcatgccttcattttgcccttttcttcagaaaataaa
gttggagcctccagaatctccaaaaaatgagctgcctctagcaccggctaaggatcttgctgaagaattgacctgccctttgtgccttgagctatttaag
gaacccgttatggtggagtgtagccacaatttctgcaaaagttgcatagagaatgcctgggaagtccggaattctgtttcctgtcctgaatgtgaagagc
ccttgcctganaagagttttatcatcaaccgaacacttgaaaactagtgatgaaaacgct
3' EST: CX966452 [+]tctctagtcatcccaatgtcccaatcagttttgaccccaacgtttaccaaccagtagtgccggcccgaatgaaatccattctttcccaacacaaaagtgt
tctgattaaagcgcaccactgagtcgaaaattttactccttttctctttatacctgattgtggtcagcccataagacagaagcagattgggatgagccgt
gtttggatctgccgttacttgttccagcattggaacgagaaagtgtttcatcttactccaaacaaaatactggataggtcctttaaatgttgcctgacaa
agttcctttgaaaccacacttctttcagtggattcaatggcagtcttttgcttctggcactcatcaatgaagctcctgatatcctttaggaagggtaagg
catccgtttcttctaacttctctttggccctgtcgattgctttcttaatgtcttcgatatcattctccaacttgatgagattactttccatttcctttaa
cagatcatccccctgcttttgcaactgatccaggagcttcttttcacgttcttccaagaatttgtgcatcttctcaaattcagatgtaatgtgttgttta
taatcaatcatcttttccttaaaaagtacaaaaaggttgtagccaaaaaacagactgaaaatgtattgaagtagatttcctgttaaagctatttaactta
gtttgtgtgttttgctgctgaaacattttgcagctatgcaggcaagcaatacaccattccgaatcattaccattcattcatatgtcctggccagagaaaa
cggtcaccaccattccatctcatggacagataatcatccgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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