XB-CLONE-3562879
Clone Name: IMAGE:7710757
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966638 [+]
agcgcatccgccctgtgttgatgatgaacaaaatggatcgtgctcttttggagctgcagttggaaccagaggagctctaccaaacttttcagcgcattgt
ggagaatgttaatgtgattatttccacctacggtgaaggagagactggacccatgggaaatatcatgattgaccctgttcttggcacagttggttttggc
tctggtctgcatggctgggctttcacactaaaacagtttgcagagatgtatgtcgcaaaatttgcagcaaagggggaaggtcagttggctcctgcagaac
gggccaaaaaagtggaggacatgatgaagaagctgtggggtgataggtattttgaccctgcaaatggaaagttcagcaagtctgcaacaaacgctgatgg
caaaaaacttcctagaaccttctgccagcttgtattggatccaattttcaaggtatttgatgcaatcatgaattttaagaaagaagaaactgccaaattg
gtcgagaaactagacatcaaacttgacagtgaggacaaagacaaagagggaaaacagttgcttaaggctgttatgagacgctggctcccagctggagagg
ctcttctgcagatgattaccatccacttgccctcaccagtgactgctcagaaataccgttgtgagctgttgtatgagggtcccccagatgatgaagctgc
catgggtatcaagaactgtgaccccaaaggccctcttatgatgtacatctcanaaatggtgcccaccactngacagggtagattttatgcctttggaaga
gttttctcaggtgtagtctccactggccctcaagtcaggataatgggaccaaactt
3' EST: CX966637 [+]gccctcgttcctgtcaaccctcaaattcacatttacttccccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagtt
atgatcatgccaatgaaagattatattcagggttccaacagaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgct
gacaggagtaaagcaaaggtttacagcttgtcaaggaagttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccactt
ggaaaggcctggtggagttatcgaaaggatctccaggtagaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaa
gtcagcagtgaaaccaaatgactcatttacaggcaggtatgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgc
tttctgttcaagacaccatagatacctcctaccacctgctcagggcactgaatttcaacaaggtagattggttccatcagacgtggctgagcagtcaaca
cacaagcatacaagacgcggcgtgcagttggaatgatctgtcctcctcctctatggatggcatcagcatgaagtgttacatcatggacatcaaaacgaac
acctctcanattttcttcacacaggacaccctcctttgtagcccactggaatcctgcaacaacgctgtctttgatttcattcagatactgaactccctta
gtaacatcagtaagtatgttgggcccagagccatcaggaccaaaacaccaaatcttcctagcttcagtcacatcccattcgtacttttcagccaagtagc
gggcacgagtcttcaactcctggcga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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