XB-CLONE-3562872
Clone Name: IMAGE:7710901
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Gene:
arhgap33
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966644 [+]
caggacgtgcactgtgtcagctctctgtgcaagttgtacttccgcgagctccccaacccgctgctcacctaccgcctgtaccagccctttactgaagcca
tgtcagcagccaccgaggaggacaaactcatccgtgtacatgacctcatccagcaactcccacccccgcactacaggacactcgagtacctgatgaaaca
tttgtcccagttaagcacccacagtgaccgtacggggatgcacgcccgcaatctggccatcatatgggctcccaacctgctgcgctcacgggacatggag
tcagtggggacgccgggggcggacgcgttccgcgaggtgcgagtgcagtcagtgctggtggagtttctgctgtgcaacgtggagaccctcttcagtgaca
cctttacttctattgcaaaagcatccggctttcgccgatcatccatgatgtccctcaggcctcggctcctcaccctccctgaagcctggtacctcatcca
gaagagagtgactgagctcagaagcccgacggatgggaaaagcagcgcaacagaggaagagcctctctctacagaaggacattccagccttgaccattga
ctcaactgaactcattttgttccacactggatgaggacaattctctgcttgatgggacagttttctctgcatgatgggaccgttttttcgtgacgcacaa
tgggacagttttcttgtgacgcatgatgggacagttttcttgtgacgcacgatgggacagttttctcgtgacgcacgatgggacagttttctcgtgacgc
acgatgggacagttttcttgtgacgcacgatgggacagtt
3' EST: CX966643 [+]acagtgtatcagtgttgggagcgaggtctctgccagagaaacatcactctcttattttattgacatttcaaaggtttcttttcactgtcaacttgtaccc
tcccaccaaaagctgaagaattacttaagatatacaatgggggatactgggaactgtcccatcatgcatcacgacaaaactgtcccatcgtgcgtcacaa
gaaaactgtcccatcgtgcgtcacaagaaaactgtcccatcgtgcgtcacaagaaaactgtcccatcgtgcgtcacgacaaaactgtcccatcgtgcgtc
acaagaaaactgtcccatcgtgcgtcacaagaaaactgtcccatcgtgcgtcacgagaaaactgtcccatcgtgcgtcacgagaaaactgtcccatcgtg
cgtcacgagaaaactgtcccatcgtgcgtcacaagaaaactgtcccatcgtgcgtcacgacaaaactgtcccatcatgcgtcacgagaaaactgtcccat
cgtgcgtcacaagaaaactgtcccatcgtgcgtcacaagaaaactgtcccatcgtgcgtcacgagaaaactgtcccatcgtgcgtcacgagaaaactgtc
ccatcgtgcgtgacaagaaaactgtcccatcatgcgtcacaagaaaactgtcccattgtgcgtcacgaaaaaacggtcccatcatgcagagaaaactgtc
ccatcaagcagagaattgtcctcatccagtgtggaacaaaatgagttcagttgagtcaatggtcaaggctggaatgtccttctgtagagagaggctcttc
ctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: