XB-CLONE-3562740
Clone Name: IMAGE:7710865
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Gene:
apob
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966735 [+]
attttgcataatgcaaacaatgaaatgataatagttaaaaataatgccatcctataagaaacaatctttttattagcttacaataacgaaaacaaatttt
tgaattgttcacacaaaaacacacatttgagggacgtgtaaaccggttgttgctactgtacttgcaaacaacacaaaagcctacgtttcagctatgccat
aatatacagcctgctcagtttttttgccagttccgaatgttctcaatgatctgctctgcagttagtttcccttcttgaagttgtctctcgataaactgct
gcagctcttcccatttctgagaacctttgtcgatgccatccagtaccatggcgcgcgcaatttctgctcgtttgcttataatgtcctctcttaaatgcgg
gatttcatcgaaggatttccagtcaaagggatgagggacttcaaccttaagttctcctttacgtgtggatatataggtcttcatttcttcagtggccgaa
ttctgaatgctttggagaagctggttgacatactctatgaaagagttgtagttttggatgataatgtcaatgagtcggcttgcctcggcaataagcttct
cgtatgaatttacaaacaggttagatgtagcttgaagtttttcattaagaatcttattggcattttgtgaaagttgcctggcagttgcagaccacttagt
tactctatcttcaagatctattgaagccctccttatgagttctttgccttgtccattagcattggtcagcaaatcgttagcatgttggtaatatttttta
acaagtcctttgtttgttctgtaatttctgc
3' EST: CX966734 [+]gagaggcttgcagtcaacagccagaggagccagtgacaaatataatgaaatcgtcaacagtgcccaaaaatggtatcaagatccaaaggatatgtcaatg
ttcttgaatgatggtgaatataaagacaaagccattgaattagtacgcgcctaccaaaataagatgaaagacttgattgatgcagctattaaatttgtga
aaaacacaagattccagttgccaggacaaagcagaaagtatacaggtgaagagctatttaaccttggtatgaaaaatgtagcgagcaacattgatgcgtt
ttttaaagccatgcaacaaatttatgatgcatgtgttcagtatattaaccgcactgagcttaaaattcccggtactgaagtaacaatcaagggcaaagaa
attacagatgcatgcaaaagctttagcgctaatctggaacgaatgaccaaaaaggccatacaggaccttcaaggcgtaagtctagaaagtgcactcaatc
agcttaaaagttttatacaggtgatcactcagaaaacagtggatatgatcaaggctttgcaaactaagaatatcgaagatgtcaaacttcagtttcagca
aatgtatagcgatgccatgaagtccgattacgcgagagaacttcagaacttagtagaagagttgaagaaagctacaagccagctccaaaagtatgcccag
accggacatgaagaactttcagaaaagttgaagcagctgcaagtctatgccaaggcactgcgtgaagaatatctagatccnaatattgtgngctggtcag
tgaagtattatgagatagaagaaaaggtcatttcactgctgcagaaaatcgttgattcactaaaggatctgtcatccccatatggaaattacactgcnga
aattacggaacaaacaaaggactttgntaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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