XB-CLONE-3562672
Clone Name: IMAGE:7710633
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Gene:
ilf3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966783 [+]
cgtcccatgcgcatctttttgaatgatgaccgccatgtcatggcaaagcactctgtggtgtatcccactcaggaggagttggaggctgtacagaacatgg
tatcccacacagagcgggccctaaaggcagtctctgactggattgatcagcaagaaaaagattgcagtggagagcaagaacaaccagagccagaagaacc
tgagacaacagaggagggcaaggacagcgaaggaaaaactggagagaatccaacaaggactctacgtggcgtgatgagggttggacttgttgccaaaggg
cttcttctgaagggagacttggaccttgaacttgtgctgttgtgcagagacaaacccacaatttctctcctaaaaagggtggcagataaccttgtcctgc
agtttgagacagtatctgaggataaatatgaagtcatacagaacataagagaagcttcaattgttatcaaaaacactaaagagccccctctgactctcaa
catccgtctcacatcccccctggtccgagaagagatggagaaactgagcgctggaggtatgggaagaagaaaacgggcagtgatcacactactaaacctt
ctcctttaaattatgcaatcagccccaccccatatctggtttggcttcagatcagtgtttgcttatttgtctcattcatagaagatcataactgcactgt
ttaaacctgcctgtgttgctgtaatacccatgtattctgggccttgttgccagctgtgttataactatctatagtggccttttgctatggttattacagt
tgtccacttgctgtgccagagagtctgctacttactgtaaagggtagcagtgccctctggctcagagtggc
3' EST: CX966782 [+]ccttttttttttttttttagttttaacacgcgtttattaaaacattatgaccacgccaaaatccaaaacaatgaatgcgactttaagacaataaaaacac
ttgtcaagatttaagtaaaaaataatagttaaaaaaaaagttataaagtgccctcaaagctatgcttcaagaactgtagagtggcttcaatttagggaaa
cgacgagtgcagtaactggccaatttaaaatctcaactgttgacattaggaaaaaaaacttccattccggcattaaaaatgtcccagggaaggttcagtc
tgccaaaattgtatacattgccaagggattaaaagtcagaaagcttgaggtgtgcgttccgagaactcagtcatgtacaagggaaagtaagagggcaagg
gaggagaaaaataaaaacaaaagtagaatgttcttggctttgtagttcaagagactggatttaaaagcttgtgggtgttgagtgtatgggtacaatactc
tagagatggtctaggcagatgcaaaatcatgataaccgtagcagtctgtgaaaaagtcactgtatcctgccgtcgcagaagtttgatagttgtagtttcc
accataacctccaccatagccaccagagttcatgtagccaccgtggtttcctccaccaaatccacctcggcctcttcctctgccgcgattcattccacct
ctaccacgacctctcatggcttgaggagggggtacctcattgtacatcattccaaaaccttggttatgctttcctgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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