XB-CLONE-3562666
Clone Name: IMAGE:7710729
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Gene:
nfyc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966787 [+]
aaaaaagatcatgaaacttgatgaagatgtgaagatgatcagtgctgaagccccagttttgtttgccaaagctgcacagatttttataacagagttaacc
ctacgtgcctggattcacacagaggacaacaaaaggagaactcttcagagaaatgacattgctatggcaatcacaaagtttgatcagtttgactttctta
ttgacattgtccccagggatgaattaaaacctccaaagcgccaggaagaagtccggcagactgtgaactccacggagccagttcagtactacttcactct
agcacagcagccagctgctgtacaagtgcaaggacagacagctggtcagcaaactacatcgtccaccaccaccctccagcccggtcagattatcatagct
cagccacagcaaggccagaatgcaccagttaccatgcaggttggagagggacagcaggtacagattgtgcaggcacagcctcagggacaaagtcagcaag
ggcagagtggagctgggcagaccatgcaagtcatgcaacaaataatcaccaacacaggagagattcagcagatcccggttcagctgaatacagggcaact
gcaatatatacgactagcccagccagtgtccggaacacaagttgttcagggacagatccagaccctaacagctaatgcacagcagattgcacaagcagat
gtccagcagggacagcagcagttcagccagtttaccgatggccagcaattgtaccagattcagcaagtcaccatgccagctgggcaagacatcgcacagc
cactgttcatccagtccagcgggcaggcatctgatggacaaactactcangtcacaggggactg
3' EST: CX966786 [+]aaacagcctgtatctaaaaagtttattgctgtctgtgcagaattgttctgtgtacacaaagcagactacagcaatacattaaaaagacaaagaaattagt
attaataataaaaactgaccccaccccccatacacaccatacattacttcctcaatagatacgtatccagtccaaatgtaatggcaaggctttatactgg
cggaacgtgcgttccggctaacacagcgctgtcagtggcaggtgtgggaggaaggctcatttaatcagatcatgaacaagagcggcaggaaatgctttgt
tactgcttcatccggaaaaggaaacggcaaaaagaattgacagcgttgctttgcaaagaagggtgccctgcggatcccaactgaaacgggttatctggtg
tttctctacaaattgtctgtggctcatctaacattgaaactcatggttcatggcctcatttattattacatttaaaagggtactatgcttttaaataaat
ggaaaatccaaatctgcatcacaagtataattagtgcaactggatgtgatcatgggaaatacactgtagggatctctcatccaggtgtttctcgtgcttc
tgttttgtcagcgtattcacaacaaaccccctccgtagaatggtcccctcacagggcaagatgcacaacagaaccaattcgggccccagctatcctgaag
gctggcactagtcatgaactgcaaacaatttccctttatccctttgagtgccagtagagcctataccacacgagtgtgtgcatttgtcggcacgtctgag
tacgtgtatatatgtgtacatgtgtctgcgttccttttcccagtcc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: