XB-CLONE-3562631
Clone Name: IMAGE:7710971
|
Gene:
wdr70
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX966810 [+]
ctccaatgagaagaagcacaagggtgtatttaaaccaagatctatgcaaggaaaacgtgtgatcccaacgtgctgcacctacagcagagatggcaaattc
attgcagctgggtgccaagatggatccatccagatctgggaccgtaatatgagtgtccacactaaattccactgtagacaagcacatactcctggcactg
atacctcctgtgtgactttttcgtatgctggtaatatccttgctactcgaggaggcgatgacaccttaaaaacatgggacattagaaagtttaagaatcc
tctcaatgtggcctctgggttagcaaactattttccaatgacagattgctgcttcagcccagacgataaactcttgataaccgggacctccgtaaagaga
ggcggtggtgatggaaagttaatgttctttgacgtagggacatttcagaaggtttatgaaatccaggtcactgaagcgagtgttgttcgctgcctttggc
acccaaagctgaaccagatcatggtgggcactggcaatggactggctaaagtatattatgatcccaacagaagccagaggggagccaagttatgtgtggt
taaaacacaacggaaggcacggcaagcagaaaccctgacgcaggattacattattacccctcatgctcttccaatgttcagagagcctcgccagagaagt
acgaggaagcagctggagaaggatcgactggacccagtgaaatcccataagccagagccacctgttgcagggccaggtcgtgggggcagagtgggtaccc
acggaggcaccttatcttcatttatcgtgaagaacattgcgctagataagactgatgacagcaacc
3' EST: CX966809 [+]ccatttgccagcgctttacagagattatacaacatttacatcagcccctgctccagaggagcttacaatctaatgtccctatcacattcacacacattat
gactgatttagtcagactctggttacactgcctgtatgtttttggagtgtggatagaaactggagaacccacaggaaacccatgcagatacagggagaac
atggctgtgcaccttgggccttaagccattcccatgggcagaaatgctgcctagatgagcactaagggtcgctgtcttgcactttagtcaggggaaagtg
taaatgggcattaattattattattattatttatatagcgctgtcagcagtctgtaacacactgagctgaggggcaacttggcaaaaagtgatctcaatg
tcccacaatcagtgtttgtagggtactttcttcaataccatttgacagcatcacagttggtcggccatgactgaaaatttgacctttagtacgtggacgg
ggcctggattagggcaatggcccgccccaattccaccccactgcacctccatcctgcccattccctctttcaccctctggttttcctgttaagcagtaat
agcaaagctgggctatgtatggcctttattgagtggctgcaacatgaattatatggaaacaatgttactgtcgatattttctgtatattcaatgcaattt
tcagaaaatgttaagagggtttttctctttatttgtcttctatgtataaaatacagtctgatatttcaaaaaaagatttagaattgggaatgggaacact
gaatgaatgctttgagctgaaactgaacgcaaaatatatgaactcttttcgtc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: