XB-CLONE-3562462
Clone Name: IMAGE:7710940
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Gene:
exosc2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966922 [+]
aattacacaacaattctcctgtagaaaagggcagcagccctgtgtttccaatgcaactttcttgggatttttagttgagtaaaactcccagcatcctcca
acagagaggcagctgccatgattcccccttccatcacgcagagaggcatcaccgcccacaacgcagaatgttcattggagcagaggggaaataaacaggg
tacttgatagaattaaggctaaaggttccatcggcaagaattaaccttcctgctccaggaggcgctgtcgggtctccatcactatgtcttctatcacctc
cactttcaatagctctttgatctggtgagccaatgaagcttcatagcaatagaggacgctggtatcatacagtaacatcttctgagaagccagggccaga
atgcagttcctcaggcgagaaatcacttcccggtcagataatgctacaggctccaggttggtgataaagcccccagcctcttcctccatctccttagggg
tagcacagagccagatgaacccattgttggccaagatgatggaggctccacagggcagattatggaagtgcgtcttgcgtctcttgatgagcgaagggga
cacccggaccagcaccccctgcccaagtttgccgtacttcaggcttcttgtgtgcagagacagtgccccatccgagtacacagcttggacctccgcgctg
atcaggtctccctcctgcaggtaatcgcgcatggccagctcgtccttagccgacctcctcctcagttcccctccaggaagattaacagcagaaagtatta
acacagagtcccaccgactattcgtatcaaccttccatctct
3' EST: CX966921 [+]ccgggggacccatcactaccgacaccggattcatgaggggccatggaacatacacagaagatgataagctggtggcttcggtggccggtgtcgttgagcg
cgtcaacaaactgatatgtgttcgagccctaaaagccaggtacaatggagaagtcggggatatagttgtcggccgtataacagaggtgcaacagaagaga
tggaaggttgatacgaatagtcggttggactctgtgttaatactttctgctgttaatcttcctggaggggaactgaggaggaggtcggctaaggacgagc
tggccatgcgcgattacctgcaggagggagacctgatcagcgcggaggtccaagctgtgtactcggatggggcactgtctctgcacacaagaagcctgaa
gtacggcaaacttgggcagggggtgctggtccgggtgtccccttcgctcatcaagagacgcaagacgcacttccataatctgccctgtggagcctccatc
atcttggccaacaatgggttcatctggctctgtgctacccctaaggagatggaggaagaggctgggggctttatcaccaacctggagcctgtagcattat
ctgaccgggaagtgatttctcgcctgaggaactgcattctggccctggcttctcagaagatgttactgtatgataccagcgtcctctattgctatgaagc
ttcattggctcaccagatcaaagagctattgaaagtggaggtgatagaagacatagtgatggagacccgacagcgcctcctggagcaggaggttaattct
tgccgatggaactttag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: