XB-CLONE-3562433
Clone Name: IMAGE:7710656
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Gene:
frat1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX966941 [+]
ctttttacaccaagccatttcctaaatgtaacaaataatgtgttccctagtcacagaatggaaataggtctagctctcctcctacatcatatacacaaga
cctactatgagcaccaatttagaccccacccccatccatggtttgcttactattcctaaccctgctcctattttgtaggtttgtaaatgttgtttaaaca
aaggctgtgagacctcagggacaagtgatcaaggatcagctactgccaattaccacaaccacatcaactgctaacaaccttgaatacattttgctatatc
cagttctacagtgggccgctcacacagaatttgtccccttttttttgataactattttggtcatgtttacaggttacttgaactatttcatacaattctc
cttgaattattgcgaaattgagctggccaagaaaatatctggtgctactagttctggcattgggcaaacgttttatttttattttggttgtacttttgga
ctataatgataaactttgctgacaagcctcgtgttgcagtttcctatgtttggttcagactgggtgcattgcaacaattcagcggcctggcacgtgcttc
tgccccaaataaacctgttgtggaatggctgcaggagtagtctgagcagggagcacatgttttcccttatttggtgttatgtaattgttctctgcttctt
gtttacaatgttcaaggggaagactggcttgtgctcatagttctctcttcctctcattatctgggagtgtttattggcactacttcttcaaggtcactcc
tgccagatcggtggcacagccctagatgataattttgttttttt
3' EST: CX966940 [+]ataagaaatcatgtttatttttattcataaatcctgagactccaggaacgcaatcaacaacacttcgttacgaagggtacactttccaacggtgccaaat
cagtcagaatttggccattggttcgagcttgtttgttagcttacacgaacaaattctcaaaaaaaaaagggacagaggtttcaaatgagagcccctctgc
taaagatgaggttggagactgactgtaaaaagagtcactgtgtcaaatcaacccacgatgcagtaagaaacaaagcaacaatatcagtcagcatcactcc
atattcacattttagccttccatatagcatagcaggcaaagaaataaggtctctctgggtttacatatacagaaacaatagtaagggggctggttaactt
ttctccttactgttgtagtagaatgtacatgaggcatggggttaatgtttgtgttttcatgatgtaaattttagtttccatcatttgtgtctcatttcac
agcttcagctaccatttaactacatgtgaaccttaaacaagcatcaaaagctcaaatcaaaatattctctggccaatgtggctaccatttaagatttaaa
aaaatgcttattcaattggtggcccagtgatggggttgtaatggcccagacactgttaaatataaataatggcattaactaaatgcaactgccacactga
gactcttaaataacatcagcatacatatgtacagtaaaactagaagtctgatgctgatttacaaaaaggttgaccttgcnaggtatgttgagctacaact
tgtaacaccaaccaacagcagaaggctgta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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